Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity. Knockdown of PKCalpha results in significant decrease of lyso-PAFAT activation after ATP stimulation | Mus musculus |
Cloned (Comment) | Organism |
---|---|
stable recombinant enzyme expression in RAW264.7 cells, recombinant expression in CHO-S-PAFR cells | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine | Mus musculus | - |
CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine | - |
? | |
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine | Mus musculus C57/BL6J | - |
CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine | - |
? | |
additional information | Mus musculus | LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine | ? | - |
? | |
additional information | Mus musculus C57/BL6J | LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8BYI6 | - |
- |
Mus musculus C57/BL6J | Q8BYI6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | stimulation by either platelet-activating factor or ATP results in PKCalpha-mediated phosphorylation of the enzyme at Ser34 causing augmentation of Vmax with minimal Km change. The enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine | - |
Mus musculus | CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine | - |
? | |
acetyl-CoA + 1-alkyl-sn-glycero-3-phosphocholine | - |
Mus musculus C57/BL6J | CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine | - |
? | |
additional information | LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine | Mus musculus | ? | - |
? | |
additional information | LPCAT2 also possesses lysophosphatidylcholine acyltransferase, EC: 2.3.1.23, activity that catalyzes the incorporation of arachidonoyl-CoA into membrane phosphatidylcholine | Mus musculus C57/BL6J | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA:lyso-PAF acetyltransferase | - |
Mus musculus |
LPCAT2 | - |
Mus musculus |
lyso-PAFAT | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
metabolism | platelet-activating factor is synthesized rapidly in the remodeling pathway from its precursor membrane phospholipid, 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine, involving the enzyme | Mus musculus |
physiological function | platelet-activating factor (PAF), a potent proinflammatory lipid mediator, is synthesized rapidly in response to extracellular stimuli by the activation of acetyl-CoA:lyso-PAF acetyltransferase, lyso-PAFAT. The enzyme is rapidly phosphorylated after methylcarbamylplatelet-activating factor stimulation to enhance its enzymatic activity | Mus musculus |