BRENDA - Enzyme Database
show all sequences of 2.3.1.60

Substrate specificity and structure-activity relationships of gentamicin acetyltransferase I. The dependence of antibiotic resistance upon substrate Vmax/Km values

Williams, J.W.; Northrop, D.B.; J. Biol. Chem. 253, 5908-5914 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-deoxystreptamine
weak
Escherichia coli
amikacin
i.e. BB-K 8
Escherichia coli
garamine
-
Escherichia coli
Kanamine
-
Escherichia coli
kanamycin A
-
Escherichia coli
kanamycin C
-
Escherichia coli
lividomycin
-
Escherichia coli
Lividomycin B
-
Escherichia coli
additional information
inhibition kinetics
Escherichia coli
neomycin
best inhibitor
Escherichia coli
paromomycin
-
Escherichia coli
Paromomycin I
-
Escherichia coli
ribostamycin
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic pattern
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
containing R-factor JR88
-
Escherichia coli C600
-
containing R-factor JR88
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0004
-
neomycin
-
Escherichia coli
0.002
-
lividomycin
-
Escherichia coli
0.0036
-
garamine
-
Escherichia coli
0.0083
-
paromomycin
-
Escherichia coli
0.024
-
amikacin
-
Escherichia coli
0.026
-
kanamycin C
-
Escherichia coli
0.07
-
ribostamycin
-
Escherichia coli
0.093
-
kanamycin A
-
Escherichia coli
0.208
-
Kanamine
-
Escherichia coli
2
-
2-deoxystreptamine
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-deoxystreptamine
weak
Escherichia coli
amikacin
i.e. BB-K 8
Escherichia coli
garamine
-
Escherichia coli
Kanamine
-
Escherichia coli
kanamycin A
-
Escherichia coli
kanamycin C
-
Escherichia coli
lividomycin
-
Escherichia coli
Lividomycin B
-
Escherichia coli
additional information
inhibition kinetics
Escherichia coli
neomycin
best inhibitor
Escherichia coli
paromomycin
-
Escherichia coli
Paromomycin I
-
Escherichia coli
ribostamycin
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0004
-
neomycin
-
Escherichia coli
0.002
-
lividomycin
-
Escherichia coli
0.0036
-
garamine
-
Escherichia coli
0.0083
-
paromomycin
-
Escherichia coli
0.024
-
amikacin
-
Escherichia coli
0.026
-
kanamycin C
-
Escherichia coli
0.07
-
ribostamycin
-
Escherichia coli
0.093
-
kanamycin A
-
Escherichia coli
0.208
-
Kanamine
-
Escherichia coli
2
-
2-deoxystreptamine
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic pattern
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + fortimicin A
-
637302
Escherichia coli
CoA + N2'-acetylfortimicin A
-
-
-
?
acetyl-CoA + gentamicin A
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin A
-
-
-
?
acetyl-CoA + gentamicin B
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B
-
-
-
?
acetyl-CoA + gentamicin B1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin B1
-
-
-
?
acetyl-CoA + gentamicin C1
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli
?
acetyl-CoA + gentamicin C1a
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
acetyl-CoA + gentamicin C1a
enzyme modifies the 3-N-position of the deoxystreptamine ring
637302
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637302
Escherichia coli C600
?
acetyl-CoA + gentamicin C2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin C2
-
-
-
?
acetyl-CoA + gentamicin XK62-2
-
637302
Escherichia coli
CoA + N3'-acetylgentamicin XK62-2
-
-
-
?
acetyl-CoA + kanamycin B
-
637302
Escherichia coli
CoA + N3'-acetylkanamycin B
-
-
-
?
acetyl-CoA + N-ethylsisomicin
-
637302
Escherichia coli
CoA + N3'-acetylethylsisomicin
-
-
-
?
acetyl-CoA + neamine
-
637302
Escherichia coli
CoA + N3'-acetylneamine
-
-
-
?
acetyl-CoA + nebramine
-
637302
Escherichia coli
CoA + N2'-acetylnebramine
-
-
-
?
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + sisomicin
best substrate
637302
Escherichia coli C600
CoA + N3'-acetylsisomicin
-
-
-
?
acetyl-CoA + tobramycin
-
637302
Escherichia coli
CoA + N3'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
-
637302
Escherichia coli C600
CoA + N3'-acetyltobramycin
-
-
-
?
additional information
substrate structure/activity relationship
637302
Escherichia coli
?
-
-
-
-
additional information
substrate structure/activity relationship
637302
Escherichia coli C600
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Other publictions for EC 2.3.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736260
Atasoy
Modifying enzymes related amin ...
Acinetobacter baumannii
Int. J. Clin. Exp. Med.
8
2874-2880
2015
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1
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1
-
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1
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1
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1
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1
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2
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1
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1
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1
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1
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-
-
-
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1
1
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-
706886
Ahmed
Genetic analysis of antimicrob ...
Escherichia coli
Vet. Microbiol.
136
397-402
2009
-
-
1
-
-
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-
-
-
-
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1
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-
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3
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4
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1
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-
3
-
-
-
-
-
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-
-
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-
1
1
-
-
-
637303
Vliegenthart
Identification of three genes ...
Escherichia coli
J. Antimicrob. Chemother.
25
759-765
1990
-
-
-
-
-
-
-
-
-
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1
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-
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-
-
-
-
-
-
-
-
-
-
637302
Williams
Substrate specificity and stru ...
Escherichia coli, Escherichia coli C600
J. Biol. Chem.
253
5908-5914
1978
-
-
-
-
-
-
13
1
-
-
-
-
-
7
-
-
-
-
-
-
-
-
21
-
-
1
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
13
10
1
-
-
-
-
-
-
-
-
-
-
-
-
21
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
637298
Williams
Purification and properties of ...
Escherichia coli, Escherichia coli C600
Biochemistry
15
125-131
1976
1
-
-
-
-
1
2
2
1
-
2
-
-
9
-
-
1
-
-
-
1
3
2
1
-
1
-
-
1
1
1
2
-
1
-
-
1
-
-
-
-
-
1
-
2
1
2
1
-
2
-
-
-
-
1
-
-
1
3
2
1
1
-
-
1
1
1
2
-
-
-
-
-
-
-