BRENDA - Enzyme Database
show all sequences of 2.3.1.60

Purification and properties of gentamicin acetyltransferase I

Williams, J.W.; Northrop, D.B.; Biochemistry 15, 125-131 (1976)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
N-methylmaleimide and DTT have no effect on activity
Escherichia coli
General Stability
General Stability
Organism
lyophilization, stable to
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
gentamicin
-
Escherichia coli
gentamicin C1a
substrate inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0003
0.0005
gentamicin C1a
-
Escherichia coli
0.0039
0.0048
acetyl-CoA
-
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
4 * 17000, SDS-PAGE
Escherichia coli
63000
-
equilibrium sedimentation centrifugation
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
containing R-factor JR88
-
Escherichia coli C600
-
containing R-factor JR88
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
20°C, lyophilized, up to 1 month
Escherichia coli
frozen, redissolved ammonium sulfate precipitate, at least 1 month
Escherichia coli
refrigerated, lyophilized, up to 1 month
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli
?
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli C600
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 17000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.57
-
N3'-acetylgentamicin C1a
-
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
8
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
irreversible inactivation
Escherichia coli
4.5
-
no activity, restorable by neutralization
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.16
-
gentamicin
-
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
N-methylmaleimide and DTT have no effect on activity
Escherichia coli
General Stability (protein specific)
General Stability
Organism
lyophilization, stable to
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
gentamicin
-
Escherichia coli
gentamicin C1a
substrate inhibition
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.16
-
gentamicin
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0003
0.0005
gentamicin C1a
-
Escherichia coli
0.0039
0.0048
acetyl-CoA
-
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
-
Escherichia coli
5737
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
4 * 17000, SDS-PAGE
Escherichia coli
63000
-
equilibrium sedimentation centrifugation
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.8
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
20°C, lyophilized, up to 1 month
Escherichia coli
frozen, redissolved ammonium sulfate precipitate, at least 1 month
Escherichia coli
refrigerated, lyophilized, up to 1 month
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli
?
acetyl-CoA + gentamicin C1a
-
637298
Escherichia coli C600
CoA + N3'-acetylgentamicin C1a
-
637298
Escherichia coli C600
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 17000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.57
-
N3'-acetylgentamicin C1a
-
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
8
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
8.5
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
irreversible inactivation
Escherichia coli
4.5
-
no activity, restorable by neutralization
Escherichia coli
Other publictions for EC 2.3.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736260
Atasoy
Modifying enzymes related amin ...
Acinetobacter baumannii
Int. J. Clin. Exp. Med.
8
2874-2880
2015
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-
1
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-
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1
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1
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1
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1
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1
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2
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1
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1
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1
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1
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1
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-
-
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1
1
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-
706886
Ahmed
Genetic analysis of antimicrob ...
Escherichia coli
Vet. Microbiol.
136
397-402
2009
-
-
1
-
-
-
-
-
-
-
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-
1
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-
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-
3
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4
-
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1
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3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
637303
Vliegenthart
Identification of three genes ...
Escherichia coli
J. Antimicrob. Chemother.
25
759-765
1990
-
-
-
-
-
-
-
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1
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637302
Williams
Substrate specificity and stru ...
Escherichia coli, Escherichia coli C600
J. Biol. Chem.
253
5908-5914
1978
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13
1
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7
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21
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1
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10
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13
10
1
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21
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1
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-
-
-
-
637298
Williams
Purification and properties of ...
Escherichia coli, Escherichia coli C600
Biochemistry
15
125-131
1976
1
-
-
-
-
1
2
2
1
-
2
-
-
9
-
-
1
-
-
-
1
3
2
1
-
1
-
-
1
1
1
2
-
1
-
-
1
-
-
-
-
-
1
-
2
1
2
1
-
2
-
-
-
-
1
-
-
1
3
2
1
1
-
-
1
1
1
2
-
-
-
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