BRENDA - Enzyme Database
show all sequences of 2.3.1.59

Aminoglycoside resistance in bacteria medicated by gentamicin acetyltransferase II, an enzyme modifying the 2-amino group of aminoglycoside antibiotics

Chevereau, M.; Daniels, P.J.L.; Davies, J.; LeGoffic, F.; Biochemistry 13, 598-603 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
activity in crude enzyme preparation is stable to repeated freezing and thawing
Providencia sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Providencia sp.
-
clinical isolate
-
Providencia sp. 164
-
clinical isolate
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + butirosin
acetylated at 30% the rate of gentamicin C1
487273
Providencia sp.
CoA + ?
-
-
-
?
acetyl-CoA + dideoxykanamycin
acetylated at 92% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-diacetyldideoxykanamycin
-
-
-
?
acetyl-CoA + gentamicin B
7% of the activity with gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamycin
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + kanamycin B
acetylated at 14% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2-acetylkanamycin B
-
-
-
?
acetyl-CoA + kanamycin C
acetylated at 40% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylkanamycin C
-
-
-
?
acetyl-CoA + neomycin B
acetylated at 10% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylneomycin
-
-
-
?
acetyl-CoA + ribostamycin
acetylated at 15% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylribostamycin
-
-
-
?
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp.
CoA + N3'-acetylsisomicin
-
-
-
-
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N3'-acetylsisomicin
-
-
-
-
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetyltobramycin
-
-
-
?
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
?
-
-
-
?
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp.
?
-
-
-
-
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp. 164
?
-
-
-
-
N-acetyl-D-glucosamine + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
D-glucosamine + N2'-acetyltobramycin
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
kanamycin C
Providencia sp.
6.6
-
gentamicin C
Providencia sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.7
6.5
about half-maximal activity at pH 5.7 and 6.5, kanamycin C
Providencia sp.
6.4
7.6
about half-maximal activity at pH 6.4 and 7.6, gentamicin C
Providencia sp.
General Stability (protein specific)
General Stability
Organism
activity in crude enzyme preparation is stable to repeated freezing and thawing
Providencia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + butirosin
acetylated at 30% the rate of gentamicin C1
487273
Providencia sp.
CoA + ?
-
-
-
?
acetyl-CoA + dideoxykanamycin
acetylated at 92% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-diacetyldideoxykanamycin
-
-
-
?
acetyl-CoA + gentamicin B
7% of the activity with gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamycin
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1a
-
-
-
?
acetyl-CoA + kanamycin B
acetylated at 14% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2-acetylkanamycin B
-
-
-
?
acetyl-CoA + kanamycin C
acetylated at 40% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylkanamycin C
-
-
-
?
acetyl-CoA + neomycin B
acetylated at 10% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylneomycin
-
-
-
?
acetyl-CoA + ribostamycin
acetylated at 15% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylribostamycin
-
-
-
?
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp.
CoA + N3'-acetylsisomicin
-
-
-
-
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N3'-acetylsisomicin
-
-
-
-
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetyltobramycin
-
-
-
?
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetyltobramycin
-
-
-
?
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
?
-
-
-
?
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp.
?
-
-
-
-
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp. 164
?
-
-
-
-
N-acetyl-D-glucosamine + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
D-glucosamine + N2'-acetyltobramycin
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
kanamycin C
Providencia sp.
6.6
-
gentamicin C
Providencia sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.7
6.5
about half-maximal activity at pH 5.7 and 6.5, kanamycin C
Providencia sp.
6.4
7.6
about half-maximal activity at pH 6.4 and 7.6, gentamicin C
Providencia sp.
Other publictions for EC 2.3.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
487274
Payie
Characterization of gentamicin ...
Providencia stuartii
J. Bacteriol.
179
4106-4114
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487275
Clarke
The prevalence of gentamicin 2 ...
no activity in Morganella morganii, no activity in Proteus myxofaciens, no activity in Proteus vulgaris, no activity in Providencia heimbachae, no activity in Providencia rustigianii, Proteus penneri, Providencia rettgeri, Providencia stuartii, Providencia stuartii PR50
FEMS Microbiol. Lett.
145
201-207
1996
-
-
-
-
-
-
-
-
-
-
-
2
-
14
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487276
Payie
The role of O-acetylation in t ...
Providencia stuartii
Microb. Drug Resist.
2
135-140
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487273
Chevereau
Aminoglycoside resistance in b ...
Providencia sp. 164, Providencia sp.
Biochemistry
13
598-603
1974
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
19
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
487272
Benveniste
Aminoglycoside antibiotic-inac ...
Streptomyces kanamyceticus, Streptomyces spectabilis
Proc. Natl. Acad. Sci. USA
70
2276-2280
1973
-
2
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-