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Literature summary for 2.3.1.57 extracted from

  • Mandal, S.; Mandal, A.; Park, M.H.
    Depletion of the polyamines spermidine and spermine by overexpression of spermidine/spermine N1-acetyltransferase 1 (SAT1) leads to mitochondria-mediated apoptosis in mammalian cells (2015), Biochem. J., 468, 435-447.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
transduction of HEK-293T cells with an adenovirus encoding the enzyme and enzyme overexpression Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 acetyl-CoA + 2 spermine Homo sapiens
-
 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
3 acetyl-CoA + spermidine Homo sapiens
-
 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P21673
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 acetyl-CoA + 2 spermine
-
 Homo sapiens 3 CoA + N1-acetylspermine + N1,N12-diacetylspermine
-
 ?
3 acetyl-CoA + spermidine
-
 Homo sapiens 3 CoA + N1,N4,N8-triacetylspermidine
-
 ?

Synonyms

Synonyms Comment Organism
SAT1
-
 Homo sapiens
spermidine/spermine N1-acetyltransferase 1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction key polyamine catabolic enzyme spermidine/spermine N1-acetyltransferase1 overexpression in HEK293T cells via adenoviral vector leads to a rapid depletion of spermidine and spermine, arrest in cell growth and a decline in cell viability. AdSAT1-transduced cells reveal morphological changes commonly associated with apoptosis, including cell shrinkage, nuclear fragmentation, mitochondrial alteration, vacuolization and membrane blebbing. As polyamine analogues, alpha-methylspermidine and N1,N12-dimethylspermine that are not substrates for SAT1 partially restore growth and prevent apoptosis of AdSAT1-transduced cells. Inhibition of polyamine oxidases does not restore the growth of AdSAT1-transduced cells or block apoptosis. AdSAT1-transduction causes apoptosis by an intrinsic mitochondrial pathway, release of cytochrome c from mitochondria to cytoplasm concomitant with a decrease in the mitochondrial fraction in AdSAT1-transduced cells Homo sapiens