Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold(DE3)pLysS cells, transformed with pGEX expression vectors | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R101K | site-directed mutagenesis of SSAT1 protein, ability to promote hypoxia-inducible factor-1 (HIF-1alpha) degradation reduced | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P21673 | encodes SSAT1 protein | - |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293 cell | - |
Homo sapiens | - |
HEK-293T cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
SSAT1 inhibits hypoxia-inducible factor-1 (HIF-1alpha) through protein/protein interaction, effect of SSAT1 loss of function on HIF-1alpha protein levels and HIF-1 transcriptional activity, mutation of Arg101 impairs SSAT1-mediated HIF-1alpha degradation, SSAT1 promotes the ubiquitination of HIF-1alpha, involvement of SSAT1 and SSAT2 in alternative pathways for ubiquitination and degradation of HIF-1alpha, complementary roles in promoting O2-independent and O2-dependent degradation of HIF-1alpha | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | roles of SSAT proteins in oxygen homeostasis, SSAT1 binding to hypoxia-inducible factor-1 (HIF-1alpha) promotes its ubiquitination/degradation, in contrast to SSAT2, SSAT1 acts by stabilizing the interaction of HIF-1alpha with RACK1 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
spermidine/spermine N1-acetyltransferase 1 | - |
Homo sapiens |
SSAT1 | - |
Homo sapiens |