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Literature summary for 2.3.1.57 extracted from

  • Haywood, G.W.; Large, P.J.
    The occurrence, subcellular localization and partial purification of diamine acetyltransferase in the yeast Candida boidinii grown on spermidine or putrescine as sole nitrogen source (1985), Eur. J. Biochem., 148, 277-283.
    View publication on PubMed
Search for more literature for 2.3.1.57

General Stability

General Stability Organism
unstable enzyme, glycerol 10% v/v or bovine serum albumin, 5 mg/ml, partially stabilizes [Candida] boidinii

Inhibitors

Inhibitors Comment Organism Structure
Butan-2,3-dione at 10 mM, in presence of borate buffer pH 9.0 [Candida] boidinii
N-ethylmaleimide
-
 [Candida] boidinii
p-chloromercuribenzoate
-
 [Candida] boidinii
Phenylglyoxal at 10 mM, in bicarbonate buffer, pH 9.0 [Candida] boidinii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 acetyl-CoA putrescine as cosubstrate [Candida] boidinii
0.26 0.44 putrescine
-
 [Candida] boidinii
0.27
-
 1,3-diaminopropane
-
 [Candida] boidinii
0.44
-
 1,5-Diaminopentane
-
 [Candida] boidinii
0.5
-
 sym-norspermidine
-
 [Candida] boidinii
1.4
-
 N8-Acetylspermidine
-
 [Candida] boidinii
1.59
-
 1,7-Diaminoheptane
-
 [Candida] boidinii
1.7
-
 spermine
-
 [Candida] boidinii
2.4
-
 spermidine
-
 [Candida] boidinii
2.4
-
 acetylputrescine
-
 [Candida] boidinii
4.4
-
 N1-acetylspermidine
-
 [Candida] boidinii

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 [Candida] boidinii 5739
-
mitochondrion
-
 [Candida] methanosorbosa 5739
-
mitochondrion
-
 Trichosporon melibiosaceum 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + putrescine [Candida] boidinii breakdown of spermidine and putrescine CoA + acetylputrescine
-
 ?
acetyl-CoA + spermidine [Candida] boidinii breakdown of spermidine and putrescine, key agent in the supply of nitrogen to the cell CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermidine [Candida] boidinii CBS 5777 breakdown of spermidine and putrescine, key agent in the supply of nitrogen to the cell CoA + N1-acetylspermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Trichosporon melibiosaceum
-
 CBS 6087
-
Trichosporon melibiosaceum CBS 6087
-
 CBS 6087
-
[Candida] boidinii
-
 CBS 5777
-
[Candida] boidinii CBS 5777
-
 CBS 5777
-
[Candida] methanosorbosa
-
 CBS 6853
-
[Candida] methanosorbosa CBS 6853
-
 CBS 6853
-

Purification (Commentary)

Purification (Comment) Organism
26fold, partial [Candida] boidinii

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine sequential mechanism [Candida] boidinii
a
acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine sequential mechanism [Candida] methanosorbosa
a
acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine sequential mechanism Trichosporon melibiosaceum
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 [Candida] boidinii
2.45
-
-
 [Candida] boidinii

Storage Stability

Storage Stability Organism
25°C, glycerol 10% v/v or bovine serum albumin, 5 mg/ml, more than 22 h, stable [Candida] boidinii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 1,3-diaminopropane
-
 [Candida] boidinii CoA + N1-acetyl-1,3-diaminopropane
-
 ?
acetyl-CoA + 1,5-diaminopentane
-
 [Candida] boidinii CoA + N1-acetyl-1,5-diaminopentane
-
 ?
acetyl-CoA + 1,7-diaminoheptane
-
 [Candida] boidinii CoA + N1-acetyl-1,7-diaminoheptane
-
 ?
acetyl-CoA + beta-phenylethylamine
-
 [Candida] boidinii CoA + N-acetylphenylethylamine
-
 ?
acetyl-CoA + histone
-
 [Candida] boidinii CoA + N-acetylhistone
-
 ?
acetyl-CoA + N-acetylputrescine
-
 [Candida] boidinii CoA + N1,N4-diacetylputrescine
-
 ?
acetyl-CoA + N-acetylputrescine
-
 [Candida] boidinii CBS 5777 CoA + N1,N4-diacetylputrescine
-
 ?
acetyl-CoA + N1-acetylspermidine low affinity [Candida] boidinii CoA + N1,N8-diacetylspermidine
-
 ?
acetyl-CoA + N1-acetylspermine
-
 [Candida] boidinii CoA + N1,N12-diacetylspermine
-
 ?
acetyl-CoA + N8-acetylspermidine
-
 [Candida] boidinii CoA + N1,N8-diacetylspermidine
-
 ?
acetyl-CoA + putrescine
-
 [Candida] boidinii CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine
-
 [Candida] methanosorbosa CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine
-
 Trichosporon melibiosaceum CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine
-
 [Candida] methanosorbosa CBS 6853 CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine
-
 [Candida] boidinii CBS 5777 CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine
-
 Trichosporon melibiosaceum CBS 6087 CoA + N-acetylputrescine
-
 ?
acetyl-CoA + putrescine breakdown of spermidine and putrescine [Candida] boidinii CoA + acetylputrescine
-
 ?
acetyl-CoA + spermidine
-
 Trichosporon melibiosaceum CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermidine
-
 [Candida] boidinii CoA + N1-acetylspermidine + N8-acetylspermidine, ratio N1 acetylspermidine:N8-acetylspermidine is 50:45 ?
acetyl-CoA + spermidine breakdown of spermidine and putrescine, key agent in the supply of nitrogen to the cell [Candida] boidinii CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermidine
-
 [Candida] boidinii CBS 5777 CoA + N1-acetylspermidine + N8-acetylspermidine, ratio N1 acetylspermidine:N8-acetylspermidine is 50:45 ?
acetyl-CoA + spermidine breakdown of spermidine and putrescine, key agent in the supply of nitrogen to the cell [Candida] boidinii CBS 5777 CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermidine
-
 Trichosporon melibiosaceum CBS 6087 CoA + N1-acetylspermidine
-
 ?
acetyl-CoA + spermine
-
 [Candida] boidinii CoA + N1-acetylspermine
-
 ?
acetyl-CoA + sym-norspermidine identical with caldine [Candida] boidinii CoA + N1-acetyl-sym-norspermidine
-
 ?
additional information not: methylamine, dimethylamine, di-n-butylamine, L-lysine, benzylamine, semicarbazide, L-serine, glyoxylate, oxaloacetate, 4-aminobenzoate, 2-aminobenzoate [Candida] boidinii ?
-
 ?
additional information not: methylamine, dimethylamine, di-n-butylamine, L-lysine, benzylamine, semicarbazide, L-serine, glyoxylate, oxaloacetate, 4-aminobenzoate, 2-aminobenzoate [Candida] boidinii CBS 5777 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at [Candida] boidinii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35.5
-
 crude extract, half-life: 0.75 min [Candida] boidinii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 putrescine [Candida] boidinii
9 9.5 spermidine [Candida] boidinii

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 7.5 stability maximum, rapid fall on either side [Candida] boidinii