General Stability | Organism |
---|---|
argon-saturated diphosphate buffer stabilizes | Rattus norvegicus |
freezing and thawing inactivates | Rattus norvegicus |
lyophilization inactivates | Rattus norvegicus |
purification inactivates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by paraoxon | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
N-Hydroxy-2-acetylaminofluorene | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
x * 38500, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | Rattus norvegicus | noninducible, no known natural substrate in rat | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | Rattus norvegicus Sprague-Dawley CD | noninducible, no known natural substrate in rat | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley CD strain | - |
Rattus norvegicus Sprague-Dawley CD | - |
Sprague-Dawley CD strain | - |
Oxidation Stability | Organism |
---|---|
unstable in the presence of oxygen due to labile SH-groups, dithiothreitol cannot fully stabilize | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | noninducible | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
14 nmol aminofluorene bound to tRNA per min per mg protein | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, up to 3 months in saturating ammonium sulfate sealed under argon | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl | - |
Rattus norvegicus | N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl | - |
? | |
N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl | - |
Rattus norvegicus Sprague-Dawley CD | N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl | - |
? | |
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | noninducible, no known natural substrate in rat | Rattus norvegicus | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? | |
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | noninducible, no known natural substrate in rat | Rattus norvegicus Sprague-Dawley CD | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38500, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
50 | - |
nucleic acid adduct formation assay | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 72 | about half-maximal activity at 25°C and 72°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.5 | Rattus norvegicus |
7 | - |
nucleic acid adduct formation assay | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.6 | 9.6 | about half-maximal activity at pH 5.6 and pH 9.6, nucleic acid adduct formation assay | Rattus norvegicus |