Literature summary for 2.3.1.54 extracted from

Zhang, Y.; Dai, Z.; Krivoruchko, A.; Chen, Y.; Siewers, V.; Nielsen, J.
Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth (2015), FEMS Yeast Res., 15, fov024.

Activating Compound

Activating Compound	Comment	Organism	Structure
reduced ferredoxin	reduced flavodoxin is te better cofactor	Escherichia coli
reduced flavodoxin	a better electron donor than reduced ferredoxin	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL.	Escherichia coli

Cloned (Comment)

Organism

gene pflB, the enzyme and its activating enzyme from Escherichia coli are expressed in a Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1. Enzyme PFL is expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively. The coexpression of either of these electron donors has a positive effect on growth under aerobic conditions, indicating increased activity of PFL.

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P09373	gene pflB	-

Synonyms

Synonyms	Comment	Organism
PFL	-	Escherichia coli
pyruvate formate lyase	-	Escherichia coli

Expression

Organism	Comment	Expression
Escherichia coli	coexpression of either of the electron donors reduced ferredoxin or reduced flavodoxin has a positive effect on growth under aerobic conditions, indicating increased activity of PFL. with a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases	up

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Release 2023.1 (January 2023)