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Literature summary for 2.3.1.54 extracted from

  • Zhou, J.; Olson, D.; Lanahan, A.; Tian, L.; Murphy, S.; Lo, J.; Lynd, L.
    Physiological roles of pyruvate ferredoxin oxidoreductase and pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum JW/SL-YS485 (2015), Biotechnol. Biofuels, 8, 138.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of the pfl deletion resulting in two different strains LL1164 and LL1170 with two different phenotypes, high lactate and low lactate, respectively. Of eight colonies picked, two have the LL1164 phenotype and six have the LL1170 phenotype. Strain LL1170 consumes more cellobiose, produces more acetate and ethanol and less lactate than strain LL1164. A pfor/pfl double deletion strain LL1178 consumes about 70% of the 5 g/L cellobiose initially present, which is about the same as its parent strain LL1141. It requires sodium acetate for growth, even in the presence of yeast extract. Lactate is the main product of fermentation Thermoanaerobacterium saccharolyticum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + formate Thermoanaerobacterium saccharolyticum
-
CoA + pyruvate
-
?
acetyl-CoA + formate Thermoanaerobacterium saccharolyticum DSM 8691
-
CoA + pyruvate
-
?

Organism

Organism UniProt Comment Textmining
Thermoanaerobacterium saccharolyticum I3VT07 gene pfl or Tsac_0628
-
Thermoanaerobacterium saccharolyticum DSM 8691 I3VT07 gene pfl or Tsac_0628
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + formate
-
Thermoanaerobacterium saccharolyticum CoA + pyruvate
-
?
acetyl-CoA + formate
-
Thermoanaerobacterium saccharolyticum DSM 8691 CoA + pyruvate
-
?

Synonyms

Synonyms Comment Organism
PFL
-
Thermoanaerobacterium saccharolyticum
pyruvate formate-lyase
-
Thermoanaerobacterium saccharolyticum

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Thermoanaerobacterium saccharolyticum

Expression

Organism Comment Expression
Thermoanaerobacterium saccharolyticum in all pyruvate ferredoxin oxidoreductase enzyme, gene pfor deletion strains, the expression levels of pyruvate formate-lyase genes are increased about sixfold compared with the parent strain. The strains show different growth dependent on the medium used up

General Information

General Information Comment Organism
metabolism the enzyme takes part in the metabolic pathway of pyruvate to ethanol in Thermoanaerobacterium saccharolyticum Thermoanaerobacterium saccharolyticum
physiological function the physiological role of pyruvate formate-lyase is supplying C1 units for biosyntheses Thermoanaerobacterium saccharolyticum