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Literature summary for 2.3.1.54 extracted from
- X-ray structure of pyruvate formate-lyase in complex with pyruvate and CoA. How the enzyme uses the Cys-418 thiyl radical for pyruvate cleavage (2002), J. Biol. Chem., 277, 40036-40042.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproducing of the enzyme in Escherichia coli 234M1 transformed with expression vector p153E1 | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| tetragonal crystals in complex with pyruvate, monoclinic cocrystals with CoA and either pyruvate or oxamate obtained by hanging drop method, space group C222_1, a = 54.90 A, b = 153.05 A, c = 205.95 | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | Escherichia coli | key enzyme of the glucose fermentation route in anaerobically growing cells | acetyl-CoA + formate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P09373 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + formate | - |
Escherichia coli | pyruvate + CoA | - |
r | |
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | key enzyme of the glucose fermentation route in anaerobically growing cells | Escherichia coli | acetyl-CoA + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFL | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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