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Literature summary for 2.3.1.54 extracted from
- Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium, Streptococcus bovis (2002), Appl. Environ. Microbiol., 68, 3352-3357.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Lactococcus lactis |
- |
Streptococcus equinus |
- |
Clostridium pasteurianum |
| recombinant pfl in Escherichia coli RM220 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Glyceraldehyde-3-phosphate | - |
Lactococcus lactis |  |
| D-Glyceraldehyde-3-phosphate | - |
Streptococcus equinus | |
| dihydroxyacetone phosphate | - |
Lactococcus lactis | |
| dihydroxyacetone phosphate | - |
Streptococcus equinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
| 87000 | - |
1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | Escherichia coli | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Lactococcus lactis | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Streptococcus equinus | - |
acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | Clostridium pasteurianum | - |
acetyl-CoA + formate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium pasteurianum | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Lactococcus lactis | - |
- |
- |
| Streptococcus equinus | - |
wild-type JB1 | - |
| Streptococcus equinus | O66391 | wild-type JB1 pfl, GenBank nucleotide sequence accession number | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Streptococcus equinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Escherichia coli | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Lactococcus lactis | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Streptococcus equinus | acetyl-CoA + formate | - |
? | |
| pyruvate + CoA | - |
Streptococcus equinus | acetyl-CoA + formate | - |
r | |
| pyruvate + CoA | - |
Clostridium pasteurianum | acetyl-CoA + formate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| dimer | - |
Clostridium pasteurianum |
| dimer | 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE | Streptococcus equinus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PFL | - |
Escherichia coli |
| PFL | - |
Lactococcus lactis |
| PFL | - |
Streptococcus equinus |
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Release 2023.1 (January 2023)
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