BRENDA - Enzyme Database show
show all sequences of 2.3.1.54

Catalytic mechanism of pyruvate formate-lyase (PFL). A theoretical study

Himo, F.; Eriksson, L.A.; J. Am. Chem. Soc. 120, 11449-11455 (1998)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + CoA
Escherichia coli
key enzyme of the glucose fermentation route in anaerobically growing cells
acetyl-CoA + formate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + CoA
-
487231
Escherichia coli
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
key enzyme of the glucose fermentation route in anaerobically growing cells
487231
Escherichia coli
acetyl-CoA + formate
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.33
-
formate
backward direction
Escherichia coli
12.8
-
pyruvate
forward direction
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyruvate + CoA
Escherichia coli
key enzyme of the glucose fermentation route in anaerobically growing cells
acetyl-CoA + formate
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pyruvate + CoA
-
487231
Escherichia coli
acetyl-CoA + formate
-
-
-
r
pyruvate + CoA
key enzyme of the glucose fermentation route in anaerobically growing cells
487231
Escherichia coli
acetyl-CoA + formate
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.33
-
formate
backward direction
Escherichia coli
12.8
-
pyruvate
forward direction
Escherichia coli
Other publictions for EC 2.3.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746477
Al-Bayati
Pneumococcal galactose catabo ...
Streptococcus pneumoniae
Sci. Rep.
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43587
2017
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735819
Zhou
Physiological roles of pyruvat ...
Thermoanaerobacterium saccharolyticum, Thermoanaerobacterium saccharolyticum DSM 8691
Biotechnol. Biofuels
8
138
2015
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736128
Zhang
Functional pyruvate formate ly ...
Escherichia coli
FEMS Yeast Res.
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2015
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744943
Zhang
Functional pyruvate formate l ...
Escherichia coli
FEMS Yeast Res.
15
fov024
2015
1
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736285
Khelifi
Anaerobic oxidation of long-ch ...
Archaeoglobus fulgidus
ISME J.
8
2153-2166
2014
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736361
Doi
Pyruvate formate-lyase is esse ...
Enterococcus faecalis, Enterococcus faecalis W11
J. Bacteriol.
196
2472-2480
2014
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736638
Doberenz
Pyruvate formate-lyase interac ...
Escherichia coli
J. Mol. Biol.
426
2827-2839
2014
1
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745316
Crain
Pyruvate formate-lyase and it ...
Escherichia coli
J. Biol. Chem.
289
5723-5729
2014
1
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727989
Crain
Pyruvate formate-lyase and its ...
Escherichia coli
J. Biol. Chem.
289
5723-5729
2013
1
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719737
Leibig
Pyruvate formate lyase acts as ...
Staphylococcus aureus
J. Bacteriol.
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952-962
2011
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701826
Wang
Metabolic Flux Control at the ...
Escherichia coli str. K-12 substr. W3110
Appl. Environ. Microbiol.
76
2107-2114
2010
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704002
Yesilkaya
Pyruvate formate lyase is requ ...
Streptococcus pneumoniae D39
Infect. Immun.
77
5418-5427
2009
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1
1
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704954
Utrilla
ATP limitation in a pyruvate f ...
Escherichia coli str. K-12 substr. MG1655
J. Ind. Microbiol. Biotechnol.
36
1057-1062
2009
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686477
Hemschemeier
Biochemical and physiological ...
Chlamydomonas reinhardtii
Eukaryot. Cell
7
518-526
2008
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1
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1
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5
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687994
Condic-Jurkic
On the modeling of arginine-bo ...
Escherichia coli
J. Comput. Chem.
29
2425-2433
2008
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671483
Meshulam-Simon
Hydrogen metabolism in Shewane ...
Shewanella oneidensis, Shewanella oneidensis MR-1 / ATCC 700550
Appl. Environ. Microbiol.
73
1153-1165
2007
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16
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671620
Nnyepi
Inactivation of E. coli pyruva ...
Escherichia coli
Arch. Biochem. Biophys.
459
1-9
2007
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672694
Yang
Computer-aided design of the s ...
Escherichia coli, Escherichia coli JM109
Biosci. Biotechnol. Biochem.
71
746-753
2007
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3
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1
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32
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672714
Zhu
The YfiD protein contributes t ...
Escherichia coli
Biotechnol. Bioeng.
97
138-143
2007
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672873
Sparling
Formate synthesis by Clostridi ...
Hungateiclostridium thermocellum
Can. J. Microbiol.
52
681-688
2006
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674782
Atteia
Pyruvate formate-lyase and a n ...
Chlamydomonas reinhardtii
J. Biol. Chem.
281
9909-9918
2006
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675371
Lehtioe
Crystal structure of a glycyl ...
Archaeoglobus fulgidus
J. Mol. Biol.
357
221-235
2006
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671437
Derzelle
Proteome analysis of Streptoco ...
Streptococcus thermophilus, Streptococcus thermophilus LMG18311
Appl. Environ. Microbiol.
71
8597-8605
2005
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674168
Lucas
Theoretical study of the suici ...
Escherichia coli
J. Am. Chem. Soc.
127
6902-6909
2005
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659056
Hasona
Pyruvate formate lyase and ace ...
Escherichia coli
J. Bacteriol.
186
7593-7600
2004
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2004
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The anaerobic chytridiomycete ...
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2003
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Biochemical and functional pro ...
Streptococcus mutans
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2
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1
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487237
Asanuma
Molecular characterization and ...
Clostridium pasteurianum, Escherichia coli, Lactococcus lactis, Streptococcus equinus
Appl. Environ. Microbiol.
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2002
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4
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4
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3
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4
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4
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9
3
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487238
Becker
X-ray structure of pyruvate fo ...
Escherichia coli
J. Biol. Chem.
277
40036-40042
2002
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1
1
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1
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3
1
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487239
Lehtioe
Structure of Escherichia coli ...
Escherichia coli
Acta Crystallogr. Sect. D
58
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2002
3
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1
1
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1
1
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487240
Melchiorsen
The level of pyruvate-formate ...
Escherichia coli, Lactococcus lactis
Appl. Microbiol. Biotechnol.
58
338-344
2002
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1
1
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2
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2
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1
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4
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1
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2
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1
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4
-
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487235
Wagner
YfiD of Escherichia coli and Y ...
Escherichia coli, Escherichia virus T4, Haemophilus influenzae, Pasteurella multocida, Salmonella enterica subsp. enterica serovar Typhimurium, Vibrio cholerae, Yersinia pestis
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285
456-462
2001
7
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1
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8
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11
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9
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8
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1
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8
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9
-
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487236
Zhang
Inactivation of pyruvate forma ...
Escherichia coli
Biochemistry
40
4123-4130
2001
-
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1
1
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1
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1
1
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2
1
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1
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1
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1
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1
1
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487234
Asanuma
Effects of pH and energy suppl ...
Clostridium butyricum, Escherichia coli, Lactococcus lactis, Streptococcus equinus, Streptococcus mutans
Appl. Environ. Microbiol.
66
3773-3777
2000
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1
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9
3
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3
5
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9
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1
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9
3
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3
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1
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1
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9
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3
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3
5
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1
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9
3
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3
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1
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661722
Plaga
Modification of Cys-418 of pyr ...
Escherichia coli
FEBS Lett.
466
45-48
2000
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1
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1
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1
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487233
Becker
Structure and mechanism of the ...
Escherichia coli
Nat. Struct. Biol.
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1999
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1
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1
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2
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4
1
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14932
Kulzer
Reconstitution and characteriz ...
Escherichia coli
J. Biol. Chem.
273
4897-4903
1998
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2
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487231
Himo
-
Catalytic mechanism of pyruvat ...
Escherichia coli
J. Am. Chem. Soc.
120
11449-11455
1998
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1
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2
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2
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487230
Broderick
-
Pyruvate formate-lyase activat ...
Escherichia coli
J. Am. Chem. Soc.
119
7396-7397
1997
1
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1
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-
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-
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14931
Weidner
Molecular characterization of ...
Clostridium butyricum, Clostridium butyricum DSM 552, Clostridium kluyveri, Clostridium pasteurianum, Clostridium pasteurianum DSM 525, Clostridium sp., Enterococcus faecalis, Escherichia coli, no activity in Clostridium pasteurianum, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, Streptococcus sanguinis DSM 20066
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1996
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13
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2
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26
2
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2
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4
13
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2
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26
2
-
-
-
-
-
-
-
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-
-
-
-
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487228
Kaiser
Pyruvate formate-lyase is not ...
Escherichia coli
FEMS Microbiol. Lett.
117
163-168
1994
-
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1
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2
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1
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1
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-
-
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2
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-
-
-
-
-
-
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487227
Knappe
-
Pyruvate formate-lyase mechani ...
Escherichia coli
Biochem. Soc. Trans.
21
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1993
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1
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1
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1
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1
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1
1
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1
1
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1
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487226
Wagner
The free radical in pyruvate f ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
89
996-1000
1992
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1
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1
1
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1
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1
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2
1
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487225
Ulissi-DeMario
-
Mechanism-based inactivation o ...
Escherichia coli
J. Am. Chem. Soc.
113
4341-4342
1991
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1
1
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-
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2
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1
1
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1
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-
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-
1
1
-
-
-
-
-
-
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-
14937
Rödel
Primary structures of Escheric ...
Escherichia coli
Eur. J. Biochem.
177
153-158
1988
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1
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1
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2
1
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2
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1
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3
1
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1
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1
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2
1
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1
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-
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3
1
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-
-
-
-
-
-
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-
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14936
Conradt
Pyruvate formate-lyase (inacti ...
Escherichia coli, Streptococcus mutans
Arch. Biochem. Biophys.
228
133-142
1984
2
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1
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1
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2
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2
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1
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1
1
3
2
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487222
Takahashi
Purification of pyruvate forma ...
Clostridium butyricum, Clostridium sp., Enterococcus faecalis, Escherichia coli, Lactobacillus delbrueckii subsp. bulgaricus, Lactococcus lactis, Streptococcus mutans, Streptococcus mutans JC2
J. Bacteriol.
149
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1982
8
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8
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1
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1
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9
2
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1
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2
1
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8
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1
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4
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2
8
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1
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3
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1
1
9
2
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1
-
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2
1
-
-
-
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-
-
-
-
487221
Knappe
-
Pyruvate formate-lyase from Es ...
Clostridium sp., Enterococcus faecalis, Escherichia coli
Methods Enzymol.
41B
508-518
1975
3
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1
1
4
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2
3
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3
1
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1
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1
1
7
1
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1
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1
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3
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1
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1
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4
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2
3
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1
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1
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1
1
7
1
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1
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1
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-
-
-
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-
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487220
Knappe
Pyruvate formate-lyase of Esch ...
Escherichia coli
Eur. J. Biochem.
50
253-263
1974
-
-
-
-
-
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1
5
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1
2
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2
1
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1
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1
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4
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-
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2
1
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1
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5
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1
2
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1
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1
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1
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4
-
-
-
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2
1
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-
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487218
Thauer
Properties and function of the ...
Clostridium beijerinckii, Clostridium butyricum, Clostridium kluyveri, Clostridium sp., Enterococcus faecalis, Escherichia coli, no activity in Clostridium pasteurianum, Veillonella parvula
Eur. J. Biochem.
27
282-290
1972
8
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25
6
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3
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12
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8
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41
-
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6
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8
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25
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6
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3
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12
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41
-
-
-
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6
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487219
Wood
Inactivation of the pyruvate f ...
Clostridium butyricum, Enterococcus faecalis, Escherichia coli
FEBS Lett.
27
49-52
1972
4
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2
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4
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6
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4
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2
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3
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6
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