Literature summary for 2.3.1.51 extracted from

Zhang, Q.; Yu, R.; Sun, D.; Bai, Z.; Li, H.; Xue, L.; Zhang, Y.; Niu, L.
PrLPAAT4, a putative lysophosphatidic acid acyltransferase from Paeonia rockii, plays an important role in seed fatty acid biosynthesis (2017), Molecules, 22, 1694-1707 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene LPAAT4, isolated from seed of unsaturated fatty acid (UFA)-rich Paeonia rockii, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of the enzyme in Arabidopsis thaliana resulting in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. AtDGAT1, AtGPAT9, and Atoleosin, involved in TAG assembly, are upregulated in PrLPAAT4-overexpressing lines. Recombinnat expression of GFP-tagged enzyme in tobacco lower epidermal cell plama membranes	Paeonia rockii

Cloned (Comment)

Organism

gene LPAAT4, isolated from seed of unsaturated fatty acid (UFA)-rich Paeonia rockii, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant overexpression of the enzyme in Arabidopsis thaliana resulting in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. AtDGAT1, AtGPAT9, and Atoleosin, involved in TAG assembly, are upregulated in PrLPAAT4-overexpressing lines. Recombinnat expression of GFP-tagged enzyme in tobacco lower epidermal cell plama membranes

Paeonia rockii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	a transmembrane enzyme, it exhibits an overlap between the transmembrane and LPLAT_AGPAT-like domains	Paeonia rockii	5886	-

Organism

Organism	UniProt	Comment	Textmining
Paeonia rockii	KX256279	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	tissue-specific expression profile of PrLPAAT4	Paeonia rockii	-
seed	-	Paeonia rockii	-

Subunits

Subunits	Comment	Organism
?	x * 42900, about, sequence calculation	Paeonia rockii

Synonyms

Synonyms	Comment	Organism
LPAAT4	-	Paeonia rockii
lysophosphatidic acid acyltransferase	-	Paeonia rockii
lysophosphatidyl acyltransferase 4	-	Paeonia rockii

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the acyl-CoA:1-acylglycerol-sn-3-phosphate acyltranferases (AGPAT) family, PrLPAAT4 possesses a 1-acyl-sn-glycerol-3-phosphate acyltransferase-related domain, it is subordinated to cluster III. Comparative analysis of gene structure and conserved domain in the LPAAT4 proteins	Paeonia rockii
metabolism	PrLPAAT4 plays an important role in seed fatty acid biosynthesis	Paeonia rockii
additional information	comparative structure deduction and analysis of PrLPAAT4	Paeonia rockii
physiological function	lysophosphatidic acid acyltransferases (LPAATs) are essential for the acylation of lysophosphatidic acid (LPA) and the synthesis of phosphatidic acid (PA), a key intermediate in the synthesis of membrane phospholipids and storage lipids. PrLPAAT4, a putative lysophosphatidic acid acyltransferase from Paeonia rockii, plays an important role in seed fatty acid biosynthesis. LPAATs play a crucial role in catalyzing the second step of triacylglycerol (TAG) formation, determining TAGs acyl composition at the sn-2-position, and controlling the conversion of LPA to PA. PrLPAAT4 might function as a positive regulator in seed fatty acid biosynthesis	Paeonia rockii