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Literature summary for 2.3.1.51 extracted from

  • Ghosh, A.K.; Chauhan, N.; Rajakumari, S.; Daum, G.; Rajasekharan, R.
    At4g24160, a soluble acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase (2009), Plant Physiol., 151, 869-881.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.51

Cloned(Commentary)

Cloned (Comment) Organism
gene At4g24160, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Arabidopsis thaliana i.e. lysophosphatidic acid CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O22975 At4g24160; gene At4g24160
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged At4g24160 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Arabidopsis thaliana CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Synonyms

Synonyms Comment Organism
acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase
-
 Arabidopsis thaliana
At4g24160
-
 Arabidopsis thaliana
LPAAT
-
 Arabidopsis thaliana
More the enzyme belongs to the alpha/beta-hydrolase family Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function LPAAT is a crucial enzyme controlling the metabolic flow of lysophosphatidic acid into the pool of phosphatidic acid, which plays a key role in many physiological aspects, such as cell signaling, cell polarity, and apoptotic signaling cascades in higher eukaryotes. Phosphatidic acid specifically regulates the genes involved in modulating cell shape and organization Arabidopsis thaliana