Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isopropyl-beta-D-thiogalactopyranoside | induction of the enzyme gene | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression in JC201 Escherichia coli cells | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
Western blotting | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | recombinant enzyme can catalyse ATP-independent acyl-CoA synthetic activity and CoA-dependent transacylation activity. Acyl-CoA synthetase is not involved in the process | Mus musculus | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
r | |
acyl-CoA + 1-O-alkyl-sn-glycerol 3-phosphate | the activity is approximately half of that toward 1-acyl-sn-glycerol 3-phosphate | Mus musculus | CoA + ? | - |
? | |
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate | - |
r | |
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
r | |
additional information | lysophospholipids such as 1-acyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphoinositol and lysoplatelet-activating factor do not serve as acyl acceptor substrates. Free fatty acids do not serve as acyl donors. The enzyme shows a broad specificity for acyl-CoAs | Mus musculus | ? | - |
? | |
additional information | reverse reaction of the enzyme: desulfo-CoA and dephospho-COA do not serve as substrates for acyl-CoA synthesis | Mus musculus | ? | - |
? | |
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Mus musculus | CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |