Cloned (Comment) | Organism |
---|---|
isolation of the JC201-plsC gene fragment containing the entire sequence of the enzyme. Complementation of the JC201 phenotype: transformation of JC201 cells with pplSC6 or pplsC6.3. Transformation of XL1-Blue cells with the control plasmid pplsC6.3, and the experimental plasmids pplsC6.3A or pplsC6.3L | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | adenine-440 is responsible for the temperature sensitive lesion in the enzyme activity of JC201. Conversion of the mutant adenine-440 back to the native guanine-440 nucleotide restores the enzyme activity at the non-permissive temperature of 42°C | Escherichia coli |
T122A | the change increases the substrate specificity in vitro for oleoyl-CoA and linoleoyl-CoA | Escherichia coli |
T122L | the change increases the substrate specificity in vitro for lignoceroyl-CoA | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
XL1-Blue cells | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
behenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-behenoyl-sn-glycerol 3-phosphate | - |
? | |
lignoceroyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-lignoceroyl-sn-glycerol 3-phosphate | - |
? | |
linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate | - |
? | |
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate | - |
? | |
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Escherichia coli |