General Stability | Organism |
---|---|
slow freezing inactivates | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MgCl2 | at high concentrations | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no Mg2+-requirement | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B | - |
Escherichia coli B / ATCC 11303 | - |
B | - |
Storage Stability | Organism |
---|---|
-15°C to 4°C, particulate preparation, fast-frozen in liquid N2, unstable | Escherichia coli |
-15°C, less than a day | Escherichia coli |
-80°C, particulate preparation, fast-frozen in liquid N2, 3 months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate | the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate | Escherichia coli | CoA + ? | - |
? | |
additional information | effect of acyl-CoA concentrations on enzyme reaction | Escherichia coli | ? | - |
? | |
additional information | 1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs | Escherichia coli | ? | - |
? | |
additional information | effect of acyl-CoA concentrations on enzyme reaction | Escherichia coli B / ATCC 11303 | ? | - |
? | |
additional information | 1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs | Escherichia coli B / ATCC 11303 | ? | - |
? | |
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | twice as good as palmitoyl-CoA | Escherichia coli | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Escherichia coli | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
7.5 | 8 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.6 | about half-maximal activity at pH 6.6 and 8.6 | Escherichia coli |