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Literature summary for 2.3.1.50 extracted from

  • Chakraborty, M.; Lou, C.; Huan, C.; Kuo, M.S.; Park, T.S.; Cao, G.; Jiang, X.C.
    Myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis (2013), J. Clin. Invest., 123, 1784-1797.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
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Mus musculus 16020
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Organism

Organism UniProt Comment Textmining
Mus musculus P97363
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-

Source Tissue

Source Tissue Comment Organism Textmining
macrophage bone marrow–derived Mus musculus
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myeloid cell
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Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + L-serine
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Mus musculus CoA + 3-dehydro-D-sphinganine + CO2
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?

Synonyms

Synonyms Comment Organism
serine palmitoyltransferase
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Mus musculus
SPT
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Mus musculus

General Information

General Information Comment Organism
malfunction myeloid cell-specific serine palmitoyltransferase subunit 2 haploinsufficiency reduces murine atherosclerosis. SPT subunit 2-haploinsufficient (Sptlc2+/-) macrophages have significantly lower SM levels in plasma membrane and lipid rafts. This reduction not only impairs inflammatory responses triggered by TLR4 and its downstream NF-kappaB and MAPK pathways, but also enhances reverse cholesterol transport mediated by ABC transporters. LDL receptor-deficient (Ldlr-/-) mice transplanted with Sptlc2+/- bone marrow cells exhibit significantly fewer atherosclerotic lesions after high-fat and high-cholesterol diet feeding. Additionally, Ldlr-/- mice with myeloid cell-specific Sptlc2 haploinsufficiency exhibit significantly less atherosclerosis than controls. Sptlc2 haploinsufficiency in macrophages leads to significant reductions of SM, glucosylceramide, and GM3 in macrophage plasma membranes and lipid rafts, resulting in altered raft distribution. Detailed phenotype overview Mus musculus
metabolism serine palmitoyltransferase is the first and rate-limiting enzyme of the de novo biosynthetic pathway of sphingomyelin. Both serine palmitoyltransferase and sphingomyelin are implicated in the pathogenesis of atherosclerosis, the development of which is driven by macrophages Mus musculus