Cloned (Comment) | Organism |
---|---|
overexpression of SPTLC3 in HEK-293 cells leads to the formation of two new sphingoid base metabolites, namely C16-sphinganine and C16-sphingosine. SPTLC3-expressing cells have higher in vitro SPT activities with lauryl- and myristoyl-CoA than SPTLC2-expressing cells, and SPTLC3 mRNA expression levels correlate closely with the C16-sphinganine synthesis rates in various human and murine cell lines. Quantitative reverse transcription--PCR analysis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the recombinant SPTLC3 subunit in HEK-293 cells, overview | Homo sapiens | |
0.03 | - |
myristoyl-CoA | pH 8.0, 37°C, recombinant SPTLC3 | Homo sapiens | |
0.04 | - |
palmitoyl-CoA | pH 8.0, 37°C, recombinant SPTLC3 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + L-serine | Homo sapiens | recombinant SPTLC3 subunit in HEK-293 cells | CoA + ? + CO2 | - |
? | |
palmitoyl-CoA + L-serine | Homo sapiens | - |
CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
SPT contains subunits SPTLC1-SPTLC3 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + L-serine | - |
Homo sapiens | CoA + ? + CO2 | - |
? | |
myristoyl-CoA + L-serine | recombinant SPTLC3 subunit in HEK-293 cells | Homo sapiens | CoA + ? + CO2 | - |
? | |
palmitoyl-CoA + L-serine | - |
Homo sapiens | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine palmitoyltransferase | - |
Homo sapiens |
SPT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | all subunits contain a pyridoxal 5'-phosphate consensus motif sequence | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | SPT catalyzes the rate-limiting step in the de novo synthesis of sphingolipids, subunit SPTLC3 generates C16-sphingoid bases, and sphingolipids with a C16 backbone constitute a significant proportion of human plasma sphingolipids | Homo sapiens |