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Literature summary for 2.3.1.50 extracted from
- Acceleration of the substrate Calpha deprotonation by an analogue of the second substrate palmitoyl-CoA in Serine Palmitoyltransferase (2008), J. Biol. Chem., 283, 7542-7553.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Sphingomonas paucimobilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA2-9SPT | mutant bearing deleted residues from Ala2 to Pro9: Km values are not significantly changed compared to wild-type | Sphingomonas paucimobilis |
| H159F | mutant enzyme shows no activity | Sphingomonas paucimobilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
palmitoyl-CoA | wild-type | Sphingomonas paucimobilis |  |
| 1.2 | - |
palmitoyl-CoA | mutant DELTA2-9SPT | Sphingomonas paucimobilis | |
| 3.5 | - |
L-serine | mutant DELTA2-9SPT | Sphingomonas paucimobilis | |
| 6.2 | - |
L-serine | wild-type | Sphingomonas paucimobilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sphingomonas paucimobilis | Q93UV0 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | using 1HNMR the exchange of the alpha-proton of L-serine with the solvent in the presence and absence of S-(2- oxoheptadecyl)-CoA, the structural analogue of palmitoyl-CoA is investagated. Results demonstrate the presence of substrate synergism, in which the alpha-proton of L-serine is activated by the binding of the second substrate palmitoyl-CoA | Sphingomonas paucimobilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + L-serine | - |
Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
| S-(2-oxoheptadecyl)-CoA + L-serine | - |
Sphingomonas paucimobilis | CoA + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| serine palmitoyltransferase | - |
Sphingomonas paucimobilis |
| SPT | - |
Sphingomonas paucimobilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sphingomonas paucimobilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Sphingomonas paucimobilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Sphingomonas paucimobilis | |
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Release 2023.1 (January 2023)
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