Application | Comment | Organism |
---|---|---|
analysis | useful as a model to elucidate the reaction mechanism | Sphingomonas paucimobilis |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination | Sphingomonas paucimobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-hydroxypropionate | competitive to L-serine | Sphingomonas paucimobilis | |
alpha-methyl-DL-serine | competitive to L-serine | Sphingomonas paucimobilis | |
cysteine | competitive to L-serine | Sphingomonas paucimobilis | |
additional information | not inhibited by halide ions | Sphingomonas paucimobilis | |
O-phosphoserine | competitive to L-serine | Sphingomonas paucimobilis | |
palmitoyl-CoA | no inhibition at concentrations up to 10 mM | Sphingomonas paucimobilis | |
serine methylester | competitive to L-serine | Sphingomonas paucimobilis | |
threonine | competitive to L-serine | Sphingomonas paucimobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.87 | - |
palmitoyl-CoA | native and recombinant enzyme | Sphingomonas paucimobilis | |
4.2 | - |
L-serine | native enzyme | Sphingomonas paucimobilis | |
10.6 | - |
L-serine | recombinant enzyme | Sphingomonas paucimobilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Sphingomonas paucimobilis | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence determination | Sphingomonas paucimobilis |
44916 | - |
2 * 44916, electrospray mass spectrometry | Sphingomonas paucimobilis |
50000 | - |
2 * 50000, SDS-PAGE | Sphingomonas paucimobilis |
90000 | - |
gel filtration | Sphingomonas paucimobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas paucimobilis | - |
- |
- |
Sphingomonas paucimobilis EY2395 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sphingomonas paucimobilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 | ordered bi-bi mechanism | Sphingomonas paucimobilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
purified enzyme | Sphingomonas paucimobilis |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl 20 mM, in sterile capped vials, 2 months without loss of activity | Sphingomonas paucimobilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidoyl-CoA + L-serine | 37% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxydocosan-3-one + CO2 | - |
? | |
elaidoyl-CoA + L-serine | 39% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxy-trans-11-eicosen-3-one + CO2 | - |
? | |
lauroyl-CoA + L-serine | 18% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxytetradecan-3-one + CO2 | - |
? | |
myristoleoyl-CoA + L-serine | 46% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxy-cis-11-hexadecen-3-one + CO2 | - |
? | |
myristoyl-CoA + L-serine | 75% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2 | - |
? | |
myristoyl-CoA + L-serine | 75% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis EY2395 | CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2 | - |
? | |
n-heptadecanoyl-CoA + L-serine | 75% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxynonadecan-3-one + CO2 | - |
? | |
n-heptadecanoyl-CoA + L-serine | 75% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis EY2395 | CoA + 2-amino-1-hydroxynonadecan-3-one + CO2 | - |
? | |
oleoyl-CoA + L-serine | 57% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxy-11-cis-eicosen-3-one + CO2 | - |
? | |
palmitoleoyl-CoA + L-serine | 80% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2 | - |
? | |
palmitoleoyl-CoA + L-serine | 80% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis EY2395 | CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2 | - |
? | |
palmitoyl-CoA + L-serine | palmitoyl-CoA is the preferred substrate | Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | no other amino acids can substitute for serine | Sphingomonas paucimobilis | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | palmitoyl-CoA is the preferred substrate | Sphingomonas paucimobilis EY2395 | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
palmitoyl-CoA + L-serine | no other amino acids can substitute for serine | Sphingomonas paucimobilis EY2395 | CoA + 3-dehydro-D-sphinganine + CO2 | - |
? | |
stearoyl-CoA + L-serine | 51% activity compared to that with palmitoyl-CoA | Sphingomonas paucimobilis | CoA + 2-amino-1-hydroxyeicosan-3-one + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Sphingomonas paucimobilis |
dimer | 2 * 44916, electrospray mass spectrometry | Sphingomonas paucimobilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sphingomonas paucimobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0388 | - |
palmitoyl-CoA | - |
Sphingomonas paucimobilis | |
0.0503 | - |
palmitoyl-CoA | recombinant enzyme | Sphingomonas paucimobilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Sphingomonas paucimobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | bound to epsilon-amino group of a lysine residue in the active site | Sphingomonas paucimobilis |