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Literature summary for 2.3.1.50 extracted from

  • Ikushiro, H.; Hayashi, H.; Kagamiyama, H.
    A water-soluble homodimeric serine palmitoyltransferase from Sphingomonas paucimobilis EY2395T strain: purification, characterization, cloning, and overproduction (2001), J. Biol. Chem., 276, 18249-18256.
    View publication on PubMed

Application

Application Comment Organism
analysis useful as a model to elucidate the reaction mechanism Sphingomonas paucimobilis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination Sphingomonas paucimobilis

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxypropionate competitive to L-serine Sphingomonas paucimobilis
alpha-methyl-DL-serine competitive to L-serine Sphingomonas paucimobilis
cysteine competitive to L-serine Sphingomonas paucimobilis
additional information not inhibited by halide ions Sphingomonas paucimobilis
O-phosphoserine competitive to L-serine Sphingomonas paucimobilis
palmitoyl-CoA no inhibition at concentrations up to 10 mM Sphingomonas paucimobilis
serine methylester competitive to L-serine Sphingomonas paucimobilis
threonine competitive to L-serine Sphingomonas paucimobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.87
-
palmitoyl-CoA native and recombinant enzyme Sphingomonas paucimobilis
4.2
-
L-serine native enzyme Sphingomonas paucimobilis
10.6
-
L-serine recombinant enzyme Sphingomonas paucimobilis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Sphingomonas paucimobilis 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence determination Sphingomonas paucimobilis
44916
-
2 * 44916, electrospray mass spectrometry Sphingomonas paucimobilis
50000
-
2 * 50000, SDS-PAGE Sphingomonas paucimobilis
90000
-
gel filtration Sphingomonas paucimobilis

Organism

Organism UniProt Comment Textmining
Sphingomonas paucimobilis
-
-
-
Sphingomonas paucimobilis EY2395
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sphingomonas paucimobilis

Reaction

Reaction Comment Organism Reaction ID
palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2 ordered bi-bi mechanism Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
purified enzyme Sphingomonas paucimobilis

Storage Stability

Storage Stability Organism
4°C, Tris-HCl 20 mM, in sterile capped vials, 2 months without loss of activity Sphingomonas paucimobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidoyl-CoA + L-serine 37% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxydocosan-3-one + CO2
-
?
elaidoyl-CoA + L-serine 39% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-trans-11-eicosen-3-one + CO2
-
?
lauroyl-CoA + L-serine 18% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxytetradecan-3-one + CO2
-
?
myristoleoyl-CoA + L-serine 46% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-cis-11-hexadecen-3-one + CO2
-
?
myristoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
?
myristoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxyhexadecan-3-one + CO2
-
?
n-heptadecanoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxynonadecan-3-one + CO2
-
?
n-heptadecanoyl-CoA + L-serine 75% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxynonadecan-3-one + CO2
-
?
oleoyl-CoA + L-serine 57% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-11-cis-eicosen-3-one + CO2
-
?
palmitoleoyl-CoA + L-serine 80% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2
-
?
palmitoleoyl-CoA + L-serine 80% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis EY2395 CoA + 2-amino-1-hydroxy-cis-11-octadecen-3-one + CO2
-
?
palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
?
palmitoyl-CoA + L-serine no other amino acids can substitute for serine Sphingomonas paucimobilis CoA + 3-dehydro-D-sphinganine + CO2
-
?
palmitoyl-CoA + L-serine palmitoyl-CoA is the preferred substrate Sphingomonas paucimobilis EY2395 CoA + 3-dehydro-D-sphinganine + CO2
-
?
palmitoyl-CoA + L-serine no other amino acids can substitute for serine Sphingomonas paucimobilis EY2395 CoA + 3-dehydro-D-sphinganine + CO2
-
?
stearoyl-CoA + L-serine 51% activity compared to that with palmitoyl-CoA Sphingomonas paucimobilis CoA + 2-amino-1-hydroxyeicosan-3-one + CO2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Sphingomonas paucimobilis
dimer 2 * 44916, electrospray mass spectrometry Sphingomonas paucimobilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sphingomonas paucimobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0388
-
palmitoyl-CoA
-
Sphingomonas paucimobilis
0.0503
-
palmitoyl-CoA recombinant enzyme Sphingomonas paucimobilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Sphingomonas paucimobilis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate bound to epsilon-amino group of a lysine residue in the active site Sphingomonas paucimobilis