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Literature summary for 2.3.1.5 extracted from
- Human Arylamine N-Acetyltransferase 1: In Vitro and Intracellular Inactivation by Nitrosoarene Metabolites of Toxic and Carcinogenic Arylamines (2008), Chem. Res. Toxicol., 21, 2005-2016.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| NAT1 is recombinantly expressed | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-nitrosofluorene | potent inactivator, incubation with 2-nitrosofluorene causes 91% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 28% | Homo sapiens |  |
| 2-nitrosotoluene | less potent inactivator of NAT1, NAT1 with 2-nitrosotoluene causes 46% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 5% | Homo sapiens | |
| 4-nitrosobenzene | less potent inactivator of NAT1, NAT1 with nitrosobenzene causes 59% inhibition of the enzyme, whereas the presence of AcCoA lowers the extent of inhibition to 13% | Homo sapiens | |
| 4-nitrosobiphenyl | potent inactivator, incubation with 4-nitrosobiphenyl causes 71% inactivation. In the presence of a 500fold excess of glutathione (0.5 mM), inhibition is reduced to 35% | Homo sapiens | |
| nitrosobenzene | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
HeLa cell NAT1, determined by SDS-PAGE and Western blot analysis | Homo sapiens |
| 37000 | - |
recombinant NAT1, determined by SDS-PAGE and Western blot analysis | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00161 | - |
HeLa cell + N-acetyl-L-cysteine + 4-nitrosobiphenyl | Homo sapiens |
| 0.00172 | - |
HeLa cell + 4-nitrosobiphenyl | Homo sapiens |
| 0.00408 | - |
in HeLa cells, substrate: p-aminosalicylic acid | Homo sapiens |
| 0.0044 | - |
in HeLa cells, substrate: p-aminobenzoic acid | Homo sapiens |
| 0.00454 | - |
HeLa cell + N-acetyl-L-cysteine | Homo sapiens |
| 0.00466 | - |
HeLa cell | Homo sapiens |
| 0.237 | - |
recombinant NAT1, substrate: p-aminobenzoic acid | Homo sapiens |
| 0.337 | - |
recombinant NAT1, substrate: p-aminosalicylic acid | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + 2-aminobenzoic acid | - |
Homo sapiens | CoA + N-acetyl-2-aminobenzoic acid | - |
? | |
| acetyl-CoA + p-aminosalicylic acid | - |
Homo sapiens | CoA + N-acetyl-4-aminosalicylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylamine N-acetyltransferase | - |
Homo sapiens |
| arylamine N-acetyltransferase 1 | - |
Homo sapiens |
| NAT1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00067 | - |
4-nitrosobiphenyl | - |
Homo sapiens | |
| 0.00067 | - |
4-nitrosobiphenyl | recombinant NAT1 | Homo sapiens | |
| 0.54 | - |
4-nitrosobenzene | - |
Homo sapiens | |
| 0.54 | - |
nitrosobenzene | recombinant NAT1 | Homo sapiens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
- |
Homo sapiens | 2-nitrosofluorene | |
| 0.00004 | - |
HeLa cell NAT1 | Homo sapiens | 2-nitrosofluorene | |
| 0.00006 | - |
- |
Homo sapiens | 4-nitrosobiphenyl | |
| 0.00006 | - |
HeLa cell NAT1 | Homo sapiens | 4-nitrosobiphenyl | |
| 0.091 | - |
- |
Homo sapiens | 2-nitrosotoluene | |
| 0.091 | - |
HeLa cell NAT1 | Homo sapiens | 2-nitrosotoluene | |
| 0.237 | - |
- |
Homo sapiens | 4-nitrosobenzene | |
| 0.237 | - |
HeLa cell NAT1 | Homo sapiens | nitrosobenzene | |
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