Cloned (Comment) | Organism |
---|---|
gene natA, DNA and amino acid sequence determination and analysis, genetic structure, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces griseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a natA disruption mutant that is catalytically inactive | Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
2-amino-4-chlorophenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
1.4 | - |
2-Aminophenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
1.9 | - |
2-amino-4-methylphenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
2 | - |
3-Amino-4-hydroxybenzoic acid | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
2.6 | - |
3-amino-4-hydroxybenzaldehyde | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
12 | - |
3-amino-4-hydroxybenzenesulfonic acid | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Streptomyces griseus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, recombinant enzyme, SDS-PAGE | Streptomyces griseus |
40000 | - |
recombinant enzyme, gel filtration | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-aminophenol | Streptomyces griseus | - |
CoA + 2-acetylaminophenol | - |
? | |
acetyl-CoA + 2-aminophenol | Streptomyces griseus IFO 13350 | - |
CoA + 2-acetylaminophenol | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | Streptomyces griseus | an intermediate of grixazone synthesis | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | Streptomyces griseus IFO 13350 | an intermediate of grixazone synthesis | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzoic acid | Streptomyces griseus | an intermediate of grixazone synthesis | CoA + 3-acetylamino-4-hydroxybenzoic acid | - |
? | |
acetyl-CoA + 5-aminosalicylic acid | Streptomyces griseus | - |
CoA + 5-acetylaminosalicylic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | A2V7M5 | gene natA | - |
Streptomyces griseus IFO 13350 | A2V7M5 | gene natA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Streptomyces griseus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + an arylamine = CoA + an N-acetylarylamine | Cys69, His108, and Asp125 form the catalytic triad | Streptomyces griseus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces griseus |
0.29 | - |
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzenesulfonic acid | Streptomyces griseus |
0.33 | - |
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzaldehyde | Streptomyces griseus |
0.46 | - |
purified recombinant enzyme, with substrate 2-amino-4-chlorophenol | Streptomyces griseus |
0.47 | - |
purified recombinant enzyme, with substrate 2-aminophenol | Streptomyces griseus |
1.1 | - |
purified recombinant enzyme, with substrate 3-amino-4-hydroxybenzoic acid or 2-amino-4-methylphenol | Streptomyces griseus |
Storage Stability | Organism |
---|---|
4°C, purified recombinant enzyme in 50 mM sodium phosphate, pH 7.0, 0.15 M NaCl, and 7.5 mM 2-mercaptoethanol, 2 weeks, stable | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 2-amino-4-chlorophenol | - |
Streptomyces griseus | CoA + 2-acetylamino-4-chlorophenol | - |
? | |
acetyl-CoA + 2-amino-4-methylphenol | best substrate | Streptomyces griseus | CoA + 2-acetylamino-4-methylphenol | - |
? | |
acetyl-CoA + 2-aminophenol | - |
Streptomyces griseus | CoA + 2-acetylaminophenol | - |
? | |
acetyl-CoA + 2-aminophenol | - |
Streptomyces griseus IFO 13350 | CoA + 2-acetylaminophenol | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | - |
Streptomyces griseus | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | an intermediate of grixazone synthesis | Streptomyces griseus | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | - |
Streptomyces griseus IFO 13350 | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzaldehyde | an intermediate of grixazone synthesis | Streptomyces griseus IFO 13350 | CoA + 3-acetylamino-4-hydroxybenzaldehyde | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzenesulfonic acid | - |
Streptomyces griseus | CoA + 3-acetylamino-4-hydroxybenzenesulfonic acid | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzoic acid | best substrate | Streptomyces griseus | CoA + 3-acetylamino-4-hydroxybenzoic acid | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzoic acid | an intermediate of grixazone synthesis | Streptomyces griseus | CoA + 3-acetylamino-4-hydroxybenzoic acid | - |
? | |
acetyl-CoA + 3-amino-4-hydroxybenzoic acid | best substrate | Streptomyces griseus IFO 13350 | CoA + 3-acetylamino-4-hydroxybenzoic acid | - |
? | |
acetyl-CoA + 4-amino-3-hydroxybenzoic acid | low activity | Streptomyces griseus | CoA + 4-acetylamino-3-hydroxybenzoic acid | - |
? | |
acetyl-CoA + 5-aminosalicylic acid | - |
Streptomyces griseus | CoA + 5-acetylaminosalicylic acid | - |
? | |
additional information | substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview | Streptomyces griseus | ? | - |
? | |
additional information | substrate specificity, product identification by LC-MS, 4-aminobenzoic acid, 3-aminobenzoic acid, and aniline are poor substrates, overview | Streptomyces griseus IFO 13350 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, recombinant enzyme, SDS-PAGE | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
NAT | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
2-amino-4-chlorophenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
0.29 | - |
3-amino-4-hydroxybenzaldehyde | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
0.41 | - |
2-Aminophenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
1.1 | - |
3-amino-4-hydroxybenzenesulfonic acid | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
1.3 | - |
3-Amino-4-hydroxybenzoic acid | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus | |
1.3 | - |
2-amino-4-methylphenol | pH 7.0, 30°C, recombinant enzyme | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces griseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Streptomyces griseus |