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Literature summary for 2.3.1.5 extracted from
- Kinetic characterization of arylamine N-acetyltransferase from Pseudomonas aeruginosa (2007), BMC Biochem., 8, 3.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-aminosalicylic acid | substrate inhibition; substrate inhibition, in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex in non-productive reaction pathways; substrate inhibition through 5-aminosalicylic acid in which the substrate binds both to the free form of the enzyme and the acetyl coenzyme A-enzyme complex | Pseudomonas aeruginosa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics analysis, kinetic mechanism, determination of the half-life of the acetylated enzyme intermediate as 270 s, overview | Pseudomonas aeruginosa | |
| 0.136 | - |
acetyl-CoA | concentration p-anisidine: 200 nM, reaction: acetyl-CoA + p-anisidine | Pseudomonas aeruginosa | |
| 0.219 | - |
5-aminosalicylic acid | - |
Pseudomonas aeruginosa | |
| 0.466 | - |
acetyl-CoA | concentration p-anisidine: 2 mM, reaction: acetyl-CoA + p-anisidine | Pseudomonas aeruginosa | |
| 1.02 | - |
acetyl-CoA | - |
Pseudomonas aeruginosa | |
| 1.02 | - |
5-aminosalicylic acid | - |
Pseudomonas aeruginosa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + an arylamine | Pseudomonas aeruginosa | catalytic pathway, overview | CoA + an N-acetylarylamine | - |
? | |
| additional information | Pseudomonas aeruginosa | the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + an arylamine = CoA + an N-acetylarylamine | ping pong reaction mechanism, kinetic mechanism, overview | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas aeruginosa |
| 0.567 | - |
substrate aniline, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 0.567 | - |
substrates: aniline and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 0.602 | - |
substrate isoniazid, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 0.602 | - |
substrates: isoniazid and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 0.629 | - |
substrate aniline, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 0.629 | - |
substrates: aniline and acetyl-CoA | Pseudomonas aeruginosa |
| 0.841 | - |
substrate p-aminobenzoic acid, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 0.841 | - |
substrates: p-aminobenzoic acid and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 1.04 | - |
substrate 5-aminosalicylic acid, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 1.04 | - |
substrates: 5-aminosalicylic acid and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 1.47 | - |
substrate 2-aminofluorene, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 1.47 | - |
substrates: 2-aminofluorene and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 2.22 | - |
substrate p-anisidine, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 2.22 | - |
substrates: p-anisidine and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 2.324 | - |
substrate isoniazid, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 2.324 | - |
substrates: isoniazid and acetyl-CoA | Pseudomonas aeruginosa |
| 2.99 | - |
substrate hydralazine, p-nitrophenylacetate as an acetyl donor | Pseudomonas aeruginosa |
| 2.99 | - |
substrates: hydralazine and p-nitrophenyl acetate | Pseudomonas aeruginosa |
| 8.2 | - |
substrate p-aminobenzoic acid, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 8.2 | - |
substrates: p-aminobenzoic acid and acetyl-CoA | Pseudomonas aeruginosa |
| 13.5 | - |
substrate p-anisidine, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 13.5 | - |
substrates: p-anisidine and acetyl-CoA | Pseudomonas aeruginosa |
| 29.55 | - |
substrate hydralazine, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 29.55 | - |
substrates: hydralazine and acetyl-CoA | Pseudomonas aeruginosa |
| 44.71 | - |
substrate 2-aminofluorene, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 44.71 | - |
substrates: 2-aminofluorene and acetyl-CoA | Pseudomonas aeruginosa |
| 73.3 | - |
substrate 5-aminosalicylic acid, AcCoA as an acetyl donor | Pseudomonas aeruginosa |
| 73.3 | - |
substrates: 5-aminosalicylic acid and acetyl-CoA | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + 2-aminofluorene | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-2-aminofluorene | - |
? | |
| 4-nitrophenyl acetate + 4-aminobenzoic acid | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
| 4-nitrophenyl acetate + 4-anisidine | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-4-anisidine | - |
? | |
| 4-nitrophenyl acetate + 5-aminosalicylic acid | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-5-aminosalicylic acid | - |
? | |
| 4-nitrophenyl acetate + aniline | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-aniline | - |
? | |
| 4-nitrophenyl acetate + hydralazine | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-hydralazine | - |
? | |
| 4-nitrophenyl acetate + isoniazid | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-isoniazid | - |
? | |
| acetyl-CoA + 2-aminofluorene | - |
Pseudomonas aeruginosa | CoA + N-acetyl-2-aminofluorene | - |
? | |
| acetyl-CoA + 2-aminofluorene | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + 5-aminosalicylic acid | - |
Pseudomonas aeruginosa | CoA + N-acetyl-5-aminosalicylic acid | - |
? | |
| acetyl-CoA + 5-aminosalicylic acid | - |
Pseudomonas aeruginosa | CoA + 5-acetylaminosalicylic acid | - |
? | |
| acetyl-CoA + an arylamine | catalytic pathway, overview | Pseudomonas aeruginosa | CoA + an N-acetylarylamine | - |
? | |
| acetyl-CoA + aniline | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + aniline | - |
Pseudomonas aeruginosa | CoA + N-acetyl-aniline | - |
? | |
| acetyl-CoA + hydralazine | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + hydralazine | - |
Pseudomonas aeruginosa | CoA + N-acetyl-hydralazine | - |
? | |
| acetyl-CoA + isoniazid | - |
Pseudomonas aeruginosa | CoA + N-acetyl-isoniazid | - |
? | |
| acetyl-CoA + isoniazid | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + p-aminobenzoic acid | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + p-aminobenzoic acid | - |
Pseudomonas aeruginosa | CoA + N-acetyl-4-aminobenzoic acid | - |
? | |
| acetyl-CoA + p-anisidine | - |
Pseudomonas aeruginosa | ? | - |
? | |
| acetyl-CoA + p-anisidine | - |
Pseudomonas aeruginosa | CoA + N-acetyl-anisidine | - |
? | |
| additional information | the enzyme is an important drug- and carcinogen metabolising enzyme that catalyses the transfer of an acetyl group from a donor, such as acetyl coenzyme A, to an aromatic or heterocyclic amine, hydrazine, hydrazide or N-hydroxylamine acceptor substrate | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | NAT acceptor substrate specificity, overview | Pseudomonas aeruginosa | ? | - |
? | |
| additional information | enzyme follows a Ping Pong Bi Bi kinetic mechanism | Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenyl acetate + 2-aminofluorene | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-2-aminofluorene | - |
? | |
| p-nitrophenyl acetate + 5-aminosalicylic acid | - |
Pseudomonas aeruginosa | p-nitrophenol + 5-acetylaminosalicylic acid | - |
? | |
| p-nitrophenyl acetate + aniline | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-aniline | - |
? | |
| p-nitrophenyl acetate + hydralazine | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-hydralazine | - |
? | |
| p-nitrophenyl acetate + isoniazid | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-isoniazid | - |
? | |
| p-nitrophenyl acetate + p-aminobenzoic acid | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-4-aminobenzoic acid | - |
? | |
| p-nitrophenyl acetate + p-anisidine | - |
Pseudomonas aeruginosa | p-nitrophenol + N-acetyl-4-anisidine | - |
? | |
| p-nitrophenylacetate + 2-aminofluorene | - |
Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenylacetate + 5-aminosalicylic acid | - |
Pseudomonas aeruginosa | 4-nitrophenol + N-acetyl-5-aminosalicylate | - |
? | |
| p-nitrophenylacetate + aniline | - |
Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenylacetate + hydralazine | - |
Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenylacetate + isoniazid | - |
Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenylacetate + p-aminobenzoic acid | - |
Pseudomonas aeruginosa | ? | - |
? | |
| p-nitrophenylacetate + p-anisidine | - |
Pseudomonas aeruginosa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylamine N-acetyltransferase | - |
Pseudomonas aeruginosa |
| arylamine N-acetyltransferase C | - |
Pseudomonas aeruginosa |
| NAT | - |
Pseudomonas aeruginosa |
| PANAT | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas aeruginosa |
| 25 | - |
activity assay | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 434 | - |
5-aminosalicylic acid | - |
Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas aeruginosa |
| 8 | - |
activity assay | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Pseudomonas aeruginosa | |
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