Cloned (Comment) | Organism |
---|---|
expression of isoform a in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with acetyl coenzyme A and histone H4 peptide, i.e. residues 1-20, to 1.9 A resolution. The cofactor and the side chain of lysine 12 of histone H4 peptide are placed in the canyon between the central and C-terminal domains. Histone H4 peptide adopts a well-defined conformation and establishes an extensive set of interactions with the enzyme including invariant residues Glu64 and Trp199, which together govern substrate-binding specificity. There is a cumulative effect of the active-site residues Glu187, Glu276, and Asp277 on deprotonation of the epsilon-amino group of reactive Lys12 for direct attack of the acetyl group of the cofactor | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D277N | strong decrease in catalytic efficiency | Homo sapiens |
D62A | 4fold increase in Km value | Homo sapiens |
E187Q | strong decrease in catalytic efficiency | Homo sapiens |
E276Q | strong decrease in catalytic efficiency | Homo sapiens |
E64A | 6fold increase in Km value | Homo sapiens |
W199A | 3fold increase in Km value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0208 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.0595 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.086 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.099 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.1 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.1267 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.197 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O14929 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H4 peptide | residues 1-20 of histone H4 | Homo sapiens | CoA + acetylpeptide of histone H4 | - |
? | |
additional information | Glu187 and Glu276 could act as the general catalytic base and together with Asp277 have a cumulative effect on deprotonation of the ?-amino group of substrate Lys12 of the histone H4 peptide, which could be mediated by water molecules | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hat1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.28 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.41 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.5 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
0.53 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.99 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
4.14 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
4.28 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
histone H4 peptide | mutant E187Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
1.5 | - |
histone H4 peptide | mutant E276Q, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
3.95 | - |
histone H4 peptide | mutant E64A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
4.88 | - |
histone H4 peptide | mutant W199A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
5.36 | - |
histone H4 peptide | mutant D277N, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
23.9 | - |
histone H4 peptide | mutant D62A, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
205 | - |
histone H4 peptide | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens | |
619 | - |
acetyl-CoA | wild-type, pH not specified in the publication, temperature not specified in the publication | Homo sapiens |