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Literature summary for 2.3.1.48 extracted from

  • Yuan, H.; Rossetto, D.; Mellert, H.; Dang, W.; Srinivasan, M.; Johnson, J.; Hodawadekar, S.; Ding, E.C.; Speicher, K.; Abshiru, N.; Perry, R.; Wu, J.; Yang, C.; Zheng, Y.G.; Speicher, D.W.; Thibault, P.; Verreault, A.; Johnson, F.B.; Berger, S.L.; Sternglanz, R.; McMahon, S.B.; Cote, J.; Marmorstein, R.
    MYST protein acetyltransferase activity requires active site lysine autoacetylation (2012), EMBO J., 31, 58-70.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens
expressed in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
the X-ray crystal structures of yeast Esa1 (yEsa1/KAT5) bound to a bisubstrate H4K16CoA inhibitor and human MOF (hMOF/KAT8/MYST1) reveal that they are autoacetylated at a strictly conserved lysine residue in MYST proteins Homo sapiens
the X-ray crystal structures of yeast Esa1 (yEsa1/KAT5) bound to a bisubstrate H4K16CoA inhibitor and human MOF (hMOF/KAT8/MYST1) reveal that they are autoacetylated at a strictly conserved lysine residue in MYST proteins Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K262Q mutant does support growth of yeast cells Saccharomyces cerevisiae
K262R mutant does not support growth of yeast cells, mutant is catalytically inactive Saccharomyces cerevisiae
K274R mutant is catalytically inactive Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Saccharomyces cerevisiae Q08649
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information acetyltransferase activity requires active site lysine autoacetylation at a conserved lysine K262 Homo sapiens
additional information acetyltransferase activity requires active site lysine autoacetylation at a conserved lysine K262 Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H4 peptide + acetyl-CoA
-
 Homo sapiens ?
-
 ?
H4 peptide + acetyl-CoA
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Esa1
-
 Saccharomyces cerevisiae
histone acetyltransferase
-
 Saccharomyces cerevisiae
hMOF
-
 Homo sapiens
MYST protein lysine acetyltransferase
-
 Homo sapiens
MYST protein lysine acetyltransferase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Homo sapiens
7.6
-
 assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
physiological function MYST protein acetyltransferase activity requires active site lysine autoacetylation Homo sapiens
physiological function MYST protein acetyltransferase activity requires active site lysine autoacetylation Saccharomyces cerevisiae