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Literature summary for 2.3.1.48 extracted from

  • Guidez, F.; Howell, L.; Isalan, M.; Cebrat, M.; Alani, R.M.; Ivins, S.; Hormaeche, I.; McConnell, M.J.; Pierce, S.; Cole, P.A.; Licht, J.; Zelent, A.
    Histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein (2005), Mol. Cell. Biol., 25, 5552-5566.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription and translation of p300 by rabbit reticulocyte extracts, transient expression of an enzyme-GAL4 reporter gene construct in 293T cells, in HeLa cells, and in KG1 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
H3-CoA-20-Tat IC50: 0.012 mM, recombinant enzyme Homo sapiens
Lys-CoA-Tat IC50: 250 nM, recombinant enzyme, complete inhibition of acetylation of the promyelotic leukemia zinc finger gene Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3 + acetyl-CoA Homo sapiens
-
 acetyl-histone H3 + CoA
-
 ?
additional information Homo sapiens the histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein ?
-
 ?
promyelotic leukemia zinc finger gene + acetyl-CoA Homo sapiens
-
 acetylated promyelotic leukemia zinc finger gene + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3 + acetyl-CoA
-
 Homo sapiens acetyl-histone H3 + CoA
-
 ?
additional information the histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein Homo sapiens ?
-
 ?
promyelotic leukemia zinc finger gene + acetyl-CoA
-
 Homo sapiens acetylated promyelotic leukemia zinc finger gene + CoA
-
 ?
promyelotic leukemia zinc finger gene + acetyl-CoA i.e. PLZF, a direct and specific substrate of the p300 HAT, no activity with the substrate deletion mutant lacking zinc fingers 6 to 9 Homo sapiens acetylated promyelotic leukemia zinc finger gene + CoA
-
 ?

Synonyms

Synonyms Comment Organism
HAT
-
 Homo sapiens
p300
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00025
-
 IC50: 250 nM, recombinant enzyme, complete inhibition of acetylation of the promyelotic leukemia zinc finger gene Homo sapiens Lys-CoA-Tat
0.012
-
 IC50: 0.012 mM, recombinant enzyme Homo sapiens H3-CoA-20-Tat