Cloned (Comment) | Organism |
---|---|
expression of the His-tagged enzyme bound in the Ada2/Ada3/Gcn5 subcomplex of SAGA or the Piccolo NuA4 complex in Escherichia coli strain BL21(DE3), polycistronic vector, method development, overview | Saccharomyces cerevisiae |
successful expression using Escherichia coli BL-21 | Saccharomyces cerevisiae |
successful expression using Escherichia coli BL-21 by using a His-tagged Ada3 subunit of the yeast Ada2/Ada3/Gcn5 complex | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE | Saccharomyces cerevisiae |
43200 | - |
x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + acetyl-CoA | Saccharomyces cerevisiae | key enzyme in post-translational modification of histones associated with transcriptionally active genes | acetyl-histone + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme in Ada2/Ada3/Gcn5 subcomplex of SAGA or in the Piccolo NuA4 complex from Escherichia coli strain BL21(DE3) by metal affinity chromatography followed by anion or cation exchange chromatography, and hydrophobicinteraction chromatography, method development, overview | Saccharomyces cerevisiae |
using talon metal affinity chromatography, anion-exchange chromatography and cation-exchange chromatography. Typical yields of the yeast Ada2/Ada3/Gcn5 complex are approximately 0.5-0.8 mg/L of culture | Saccharomyces cerevisiae |
using talon metal affinity chromatography, anion-exchange chromatography, cation-exchange chromatography and hydrophobic interaction chromatography. Typical yields of the Piccolo NuA4 complex are of the 1-3 mg per liter of culture | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone + acetyl-CoA | key enzyme in post-translational modification of histones associated with transcriptionally active genes | Saccharomyces cerevisiae | acetyl-histone + CoA | - |
? | |
histone H2A + acetyl-CoA | acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview | Saccharomyces cerevisiae | acetyl-histone H2A + CoA | - |
? | |
histone H2B + acetyl-CoA | acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview | Saccharomyces cerevisiae | acetyl-histone H2B + CoA | - |
? | |
histone H3 + acetyl-CoA | acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview | Saccharomyces cerevisiae | acetyl-histone H3 + CoA | - |
? | |
histone H4 + acetyl-CoA | acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview | Saccharomyces cerevisiae | acetyl-histone H4 + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, recombinant Ada2/Ada3/Gcn5-enzyme complex, SDS-PAGE, x * 43200, recombinant Piccolo NuA4-enzyme complex, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Ada2/Ada3/Gcn5 complex | - |
Saccharomyces cerevisiae |
HAT | - |
Saccharomyces cerevisiae |
histone acetyltransferase | - |
Saccharomyces cerevisiae |
Piccolo NuA4 complex | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Saccharomyces cerevisiae |