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Literature summary for 2.3.1.48 extracted from

  • Karanam, B.; Jiang, L.; Wang, L.; Kelleher, N.L.; Cole, P.A.
    Kinetic and mass spectrometric analysis of p300 histone acetyltransferase domain autoacetylation (2006), J. Biol. Chem., 281, 40292-40301.
    View publication on PubMed
Search for more literature for 2.3.1.48

Inhibitors

Inhibitors Comment Organism Structure
Lys-CoA autoacetylation IC50: 100 nM, below Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, site-specific kinetic analysis of loop autoacetylation, PCAF-mediated autoacetylation, overview Homo sapiens
0.0033
-
 acetyl-CoA autoacetylation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone + acetyl-CoA Homo sapiens
-
 acetyl-histone + CoA
-
 ?
additional information Homo sapiens acetylation of proteins by the enzyme plays a critical role in the regulation of gene expression ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information p300 performs autoacetylation e.g. at Lys1555, Lys1554, Lys1552, and Lys1546, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone + acetyl-CoA
-
 Homo sapiens acetyl-histone + CoA
-
 ?
additional information acetylation of proteins by the enzyme plays a critical role in the regulation of gene expression Homo sapiens ?
-
 ?
additional information in absence of acceptor substrate the enzyme performs autoacetylation, identification of 13 autoacetylation sites and mechanism, autoacetylation catalyzed by p300 HAT is about 1000-fold more efficient than p300/CREB-binding protein-associated factor-mediated acetylation of catalytically defective p300 HAT, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
p300 HAT
-
 Homo sapiens
p300 histone acetyltransferase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 Lys-CoA inhibition of peptide acetylation Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0001
-
 autoacetylation IC50: 100 nM, below Homo sapiens Lys-CoA