Any feedback?
Literature summary for 2.3.1.48 extracted from
- Kinetic mechanism of human histone acetyltransferase P/CAF (2000), Biochemistry, 39, 11961-11969.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the catalytic domain of PCAF protein, amino acid residues 493-658, in Escherichia coli BL21 (DE3) | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetylated histone H3 peptide | acetylated at Lys14, product inhibition of PCAF protein, noncompetitive against both substrates | Homo sapiens | |
| CoA | product inhibition of PCAF protein, competitive against acetyl-CoA | Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
PCAF protein | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant catalytic domain of PCAF protein from E. coli | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine | PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + histone | - |
Homo sapiens | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone H3 | Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain | Homo sapiens | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3 peptide | Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain | Homo sapiens | CoA + acetylhistone H3 peptide | - |
? | |
| acetyl-CoA + histone H3-peptide | - |
Homo sapiens | CoA + acetylhistone H3 -peptide | - |
? | |
| additional information | Gcn5 and PCAF protein are transcription cofactors | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAT | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0383 | - |
acetyl-CoA | PCAF catalytic domain | Homo sapiens | |
| 0.0383 | - |
histone H3-peptide | PCAF catalytic domain | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
reaction kinetic is pH-dependent | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | PCAF protein | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00044 | - |
coenzyme A | PCAF catalytic domain | Homo sapiens | |
| 0.00066 | - |
acetylated histone H3 peptide | PCAF catalytic domain | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
