BRENDA - Enzyme Database show
show all sequences of 2.3.1.47

Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase

Fan, S.; Li, D.F.; Wang, D.C.; Fleming, J.; Zhang, H.; Zhou, Y.; Zhou, L.; Zhou, J.; Chen, T.; Chen, G.; Zhang, X.E.; Bi, L.; Int. J. Biochem. Cell Biol. 58, 71-80 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
the enzyme is a target for development of drugs against mycobac-terial pathogens
Mycolicibacterium smegmatis
Cloned(Commentary)
Commentary
Organism
gene bioF, the enzyme coding gene is located between genes bioA(MSMEG 3188) and bioD (MSMEG 3190), recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3)
Mycolicibacterium smegmatis
Crystallization (Commentary)
Crystallization
Organism
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pimeloyl-[acyl-carrier protein] + L-alanine
Mycolicibacterium smegmatis
-
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
Mycolicibacterium smegmatis ATCC 700084
-
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mycolicibacterium smegmatis
A0QX65
gene bioF
-
Mycolicibacterium smegmatis ATCC 700084
A0QX65
gene bioF
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pimeloyl-[acyl-carrier protein] + L-alanine
-
736245
Mycolicibacterium smegmatis
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
-
736245
Mycolicibacterium smegmatis ATCC 700084
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
the structure reveals an incomplete dimer, three-dimensional structure of apoenzyme, overview
Mycolicibacterium smegmatis
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
dependent on, binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo
Mycolicibacterium smegmatis
Application (protein specific)
Application
Commentary
Organism
drug development
the enzyme is a target for development of drugs against mycobac-terial pathogens
Mycolicibacterium smegmatis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bioF, the enzyme coding gene is located between genes bioA(MSMEG 3188) and bioD (MSMEG 3190), recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3)
Mycolicibacterium smegmatis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
dependent on, binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo
Mycolicibacterium smegmatis
Crystallization (Commentary) (protein specific)
Crystallization
Organism
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pimeloyl-[acyl-carrier protein] + L-alanine
Mycolicibacterium smegmatis
-
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
Mycolicibacterium smegmatis ATCC 700084
-
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
pimeloyl-[acyl-carrier protein] + L-alanine
-
736245
Mycolicibacterium smegmatis
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
pimeloyl-[acyl-carrier protein] + L-alanine
-
736245
Mycolicibacterium smegmatis ATCC 700084
8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
the structure reveals an incomplete dimer, three-dimensional structure of apoenzyme, overview
Mycolicibacterium smegmatis
General Information
General Information
Commentary
Organism
metabolism
the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid
Mycolicibacterium smegmatis
additional information
the structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS). Pyridoxal 5'-phosphate binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo, structure homology modeling and structure-function analysis of MsBioF, overview
Mycolicibacterium smegmatis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is essential for mycobacterial growth and plays a key role as the gatekeeper of the biotin biosynthesis pathway, catalyzing the first committed step, decarboxylative condensation of L-alanine with pimeloyl-CoA to form 7-oxo-8-aminopelargonic acid
Mycolicibacterium smegmatis
additional information
the structure reveals an incomplete dimer, and the active site organization is similar to, but distinct from Escherichia coli 8-amino-7-oxononanoate synthase (EcAONS). Pyridoxal 5'-phosphate binding site residues His129, Lys235, and His200 are essential for MsBioF activity in vivo and residue Glu171 plays an important, but not essential role in MsBioF activity. The N-terminus (residues 1-37) is also essential for MsBioF activity in vivo, structure homology modeling and structure-function analysis of MsBioF, overview
Mycolicibacterium smegmatis
Other publictions for EC 2.3.1.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736245
Fan
Structure and function of Myco ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Int. J. Biochem. Cell Biol.
58
71-80
2015
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1
1
1
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2
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736946
Hahn
Triazolyl phenyl disulfides: 8 ...
Arabidopsis thaliana
Pestic. Biochem. Physiol.
125
78-83
2015
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1
1
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-
8
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1
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3
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1
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1
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8
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735394
Fan
Purification and X-ray crystal ...
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis ATCC 700084
Acta Crystallogr. Sect. F
70
1372-1375
2014
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1
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1
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1
1
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719127
Kubota
Detection and characterization ...
Aquifex aeolicus, Escherichia coli, Geobacillus kaustophilus, Geobacillus kaustophilus JCM 12893, Hungateiclostridium thermocellum, Hungateiclostridium thermocellum ATCC 27405, Symbiobacterium thermophilum, Symbiobacterium thermophilum IAM14863, Thermosynechococcus elongatus
Biosci. Biotechnol. Biochem.
76
685-690
2012
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6
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8
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6
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24
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15
6
6
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6
6
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8
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6
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6
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15
6
6
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6
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7
7
719203
Cho
-
The identification of binding ...
Arabidopsis thaliana
Bull. Korean Chem. Soc.
33
1597-1602
2012
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17
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17
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17
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1
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718696
Turbeville
Functional asymmetry for the a ...
Escherichia coli
Arch. Biochem. Biophys.
511
107-117
2011
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1
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4
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1
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2
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4
4
720623
Hwang
-
Validation of 7-keto-8-aminope ...
Arabidopsis thaliana
Pestic. Biochem. Physiol.
97
24-31
2010
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1
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702483
Mann
Inhibition of 7,8-diaminopelar ...
Escherichia coli, Escherichia coli C268, Mycobacterium tuberculosis
Biochimie
91
826-834
2009
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2
2
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2
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6
2
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673041
Kerbarh
Mechanism of alpha-oxoamine sy ...
Escherichia coli
Chem. Commun. (Camb. )
2006
60-62
2006
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674626
Bhor
Broad substrate stereospecific ...
Mycobacterium tuberculosis
J. Biol. Chem.
281
25076-25088
2006
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1
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4
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1
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6
1
1
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3
1
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676274
Alexeev
Suicide inhibition of alpha-ox ...
Escherichia coli
Org. Biomol. Chem.
4
1209-1212
2006
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1
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1
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676580
Pinon
Biotin synthesis in plants. Th ...
Arabidopsis thaliana
Plant Physiol.
139
1666-1676
2005
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1
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5
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1
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487058
Webster
Mechanism of 8-amino-7-oxonona ...
Escherichia coli
Biochemistry
39
516-528
2000
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1
1
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1
2
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1
2
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2
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1
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1
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1
1
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487054
Ploux
Slow-binding and competitive i ...
Lysinibacillus sphaericus
Eur. J. Biochem.
259
63-70
1999
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1
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5
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1
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487056
Webster
Characterization of 8-amino-7- ...
Escherichia coli, Lysinibacillus sphaericus
Biochem. Soc. Trans.
26
S268
1998
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1
1
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2
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1
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1
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487057
Alexeev
The crystal structure of 8-ami ...
Escherichia coli
J. Mol. Biol.
284
401-419
1998
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487053
Ploux
Mechanistic studies on the 8-a ...
Lysinibacillus sphaericus
Eur. J. Biochem.
236
301-308
1996
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1
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487055
Spinelli
Crystallization and preliminar ...
Lysinibacillus sphaericus
Acta Crystallogr. Sect. D
52
866-868
1996
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1
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487048
Ploux
The 8-amino-7-oxopelargonate s ...
Lysinibacillus sphaericus
Biochem. J.
283
327-331
1992
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1
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3
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2
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3
2
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6
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1
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1
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2
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1
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2
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1
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1
1
2
1
1
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1
2
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487049
Otsuka
The Escherichia coli biotin bi ...
Escherichia coli
J. Biol. Chem.
263
19577-19585
1988
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2
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2
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-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1333
Izumi
-
Characterization of biotin bio ...
Lipomyces starkeyi, Lysinibacillus sphaericus, Lysinibacillus sphaericus IFO 3525, Sporidiobolus salmonicolor, Sporobolomyces coprophilus
Agric. Biol. Chem.
45
1983-1989
1981
-
-
-
-
-
-
-
-
-
1
-
9
-
9
-
-
-
-
-
-
4
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
4
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487051
Izumi
-
Distribution of 7-keto-8-amino ...
Alcaligenes faecalis, Bacillus subtilis, Citrobacter freundii, Enterobacter cloacae, Erwinia aroidea, Escherichia coli, Klebsiella aerogenes, Kocuria rosea, Lysinibacillus sphaericus, Pseudomonas fluorescens
Agric. Biol. Chem.
37
1335-1340
1973
2
-
-
-
-
-
25
-
-
-
-
20
-
10
-
-
1
-
-
-
11
-
23
-
-
-
-
-
-
-
-
10
6
-
-
2
-
-
10
-
-
-
-
25
6
-
-
-
-
20
-
-
-
1
-
-
11
-
23
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487052
Izumi
-
Partial purification and some ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
37
1327-1333
1973
-
-
-
-
-
-
21
-
-
1
-
2
-
1
-
-
1
-
-
-
1
1
3
-
2
1
3
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
21
-
-
-
1
-
2
-
-
-
1
-
-
1
1
3
-
2
1
3
-
1
1
-
-
-
-
-
-
-
-
487047
Eidenberg
Synthesis of 7-oxo-8-aminopela ...
Escherichia coli
J. Bacteriol.
96
1291-1297
1968
-
-
-
-
1
-
2
-
-
-
-
2
-
1
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-