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Literature summary for 2.3.1.47 extracted from

  • Ploux, O.; Breyne, O.; Carillon, S.; Marquet, A.
    Slow-binding and competitive inhibition of 8-amino-7-oxopelargonate synthase, a pyridoxal-5'-phosphate-dependent enzyme involved in biotin biosynthesis, by substrate and intermediate analogs: kinetic and binding studies (1999), Eur. J. Biochem., 259, 63-70.
    View publication on PubMed
Search for more literature for 2.3.1.47

Application

Application Comment Organism
agriculture possible target for design of inhibitors as herbicides and antibacterial agents Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
2-amino-3-hydroxy-2-methylnonadioic acid competitive, transition state analogue Lysinibacillus sphaericus
4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate competitive, transition state analogue Lysinibacillus sphaericus
8-amino-7-oxo-8-phosphonononanoic acid strong inhibition Lysinibacillus sphaericus
D-alanine competitive; Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site Lysinibacillus sphaericus
additional information inhibition mechanism; no inhibition by 8-amino-7-oxo-8-phosphonononanoic acid Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus second step in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Reaction

Reaction Comment Organism Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] binding study of L-alanine and D-alanine to the enzyme Lysinibacillus sphaericus
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site Lysinibacillus sphaericus
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] binding of pimeloyl-CoA causes a conformational change in the active site Lysinibacillus sphaericus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine second step in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate Schiff base formation between D- or L-alanine and pyridoxal-5'-phophate in the active site Lysinibacillus sphaericus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
 8-amino-7-oxo-8-phosphonononanoic acid
-
 Lysinibacillus sphaericus
0.068
-
 4-carboxybutyl(1-amino-1-carboxyethyl)phosphonate
-
 Lysinibacillus sphaericus
0.08
-
 2-amino-3-hydroxy-2-methylnonadioic acid
-
 Lysinibacillus sphaericus
0.59
-
 D-alanine
-
 Lysinibacillus sphaericus