Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.47 extracted from

  • Ploux, O.; Marquet, A.
    Mechanistic studies on the 8-amino-7-oxopelargonate synthase, a pyridoxal-5'-phosphate-dependent enzyme involved in biotin biosynthesis (1996), Eur. J. Biochem., 236, 301-308.
    View publication on PubMed
Search for more literature for 2.3.1.47

Cloned(Commentary)

Cloned (Comment) Organism
improved overexpression in Escherichia coli bioF- strain R874 Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 pimeloyl-CoA
-
 Lysinibacillus sphaericus
0.002
-
 L-alanine
-
 Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus second step in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Reaction

Reaction Comment Organism Reaction ID
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism Lysinibacillus sphaericus
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism, formation of the pyridoxal-phosphate-stabilized carbanion by abstraction of the C2-H proton of the alanine-pyridoxal-phosphate aldimine, followed by acylation and decarboxylation Lysinibacillus sphaericus
a
pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism could be shared by all alpha-oxoamine synthases Lysinibacillus sphaericus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine second step in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 pimeloyl-CoA
-
 Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Lysinibacillus sphaericus
pyridoxal 5'-phosphate incubation of enzyme with L-alanine but without pyridoxal-5'-phosphate lead to abortive transamination Lysinibacillus sphaericus