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Literature summary for 2.3.1.45 extracted from

  • Mandal, C.; Srinivasan, G.V.; Chowdhury, S.; Chandra, S.; Mandal, C.; Schauer, R.; Mandal, C.
    High level of sialate-O-acetyltransferase activity in lymphoblasts of childhood acute lymphoblastic leukaemia (ALL): enzyme characterization and correlation with disease status (2008), Glycoconj. J., 26, 57-73.
    View publication on PubMed
Search for more literature for 2.3.1.45

Application

Application Comment Organism
analysis the non-radioactive ELISA can quickly detect sialate-O-acetyltransferase, and thus, may become a suitable tool for acute lymphoblastic leukemia-monitoring in larger scale Homo sapiens
medicine high level of SOAT activity in lymphoblasts of childhood acute lymphoblastic leukaemia (ALL) is detected. SOAT in cell lysates or microsomal fractions of lymphoblasts of patients is up to 22fold higher than in healthy donors. SOAT activity increases at the diagnosis of leukaemia, decreases with clinical remission and sharply increases again in relapsed patients as determined by radiometric-assay. A newly developed non-radioactive ELISA can quickly detect SOAT, and serves as tool for ALL-monitoring in larger scale Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CMP
-
 Homo sapiens
coenzyme A
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00292
-
 acetyl-CoA average Km value of patients suffering from acute lymphoblastic leukaemia, Vmax: 21.04 pmol/mg/min Homo sapiens
0.00316
-
 acetyl-CoA average Km value of healthy patients, Vmax: 6.32 pmol/mg/min Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi vesicle
-
 Homo sapiens
-
-
microsome
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
 Homo sapiens
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + N-acetylneuraminate Homo sapiens
-
 CoA + N-acetyl-7-O-acetylneuraminate
-
 ?
acetyl-CoA + N-acetylneuraminate Homo sapiens
-
 CoA + N-acetyl-9-O-acetylneuraminate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
acute lymphoblastic leukemia cell line
-
 Homo sapiens
-
acute lymphoblastic leukemia cell line ALL-cell line Homo sapiens
-
lymphoblast
-
 Homo sapiens
-
lymphoblast from bone marrow Homo sapiens
-
lymphoblastic leukemia cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + CMP-N-acetylneuraminic acid
-
 Homo sapiens CoA + CMP-N-acetyl-7-O-acetylneuraminic acid
-
 ?
acetyl-CoA + endogenous sialic acid
-
 Homo sapiens CoA + N-acetyl-7-O-acetylneuraminic acid
-
 ?
acetyl-CoA + ganglioside GD3
-
 Homo sapiens CoA + ganglioside 9-O-acetyl-GD3
-
 ?
acetyl-CoA + N-acetylneuraminate
-
 Homo sapiens CoA + N-acetyl-7-O-acetylneuraminate
-
 ?
acetyl-CoA + N-acetylneuraminate
-
 Homo sapiens CoA + N-acetyl-9-O-acetylneuraminate
-
 ?

Synonyms

Synonyms Comment Organism
sialate O-acetyltransferase
-
 Homo sapiens
sialate-O-acetyltransferase
-
 Homo sapiens
SOAT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 activity assay Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57
-
 30 min, 75% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 radiometric assay using endogenous substrate, CMP-Neu5Ac, ganglioside GD3 and 14C-AcCoA Homo sapiens
7
-
 assay at Homo sapiens
7
-
 radiometric assay using endogenous substrate, glycoproteins and 3H-AcCoA Homo sapiens
7.2
-
 ELISA Homo sapiens

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens sialate-O-acetyltransferase activity decreases with clinical remission down
Homo sapiens sialate-O-acetyltransferase activity increases at the diagnosis of leukemia, sharply increases again in relapsed patients up