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Literature summary for 2.3.1.45 extracted from

  • Houliston, R.S.; Endtz, H.P.; Yuki, N.; Li, J.; Jarrell, H.C.; Koga, M.; van Belkum, A.; Karwaski, M.F.; Wakarchuk, W.W.; Gilbert, M.
    Identification of a sialate O-acetyltransferase from Campylobacter jejuni: demonstration of direct transfer to the C-9 position of terminal alpha-2,8-linked sialic acid (2006), J. Biol. Chem., 281, 11480-11486.
    View publication on PubMed
Search for more literature for 2.3.1.45

Cloned(Commentary)

Cloned (Comment) Organism
orf11 gene, DNA and amino acid sequence determination and analysis, expression as soluble enzyme in Escherichia coli strain AD202 Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + N-acetylneuraminic acid Campylobacter jejuni
-
 CoA + N-acetyl-9-O-acetyl-neuraminate no 7-O-acetylation ?
additional information Campylobacter jejuni the enzyme is involved in the lipo-oligosaccharide biosynthesis, and in production of sialylated outer core structures that mimic host gangliosides, implicated in triggering the onset of Guillain-Barré syndrome ?
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q9L9Q2 strain ATCC 43446; strain ATCC 43446
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain AD202 by affinity chromatography Campylobacter jejuni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, overview Campylobacter jejuni
0.0004
-
 purified recombinant enzyme, substrate monosialylated acceptor Campylobacter jejuni
0.0172
-
 purified recombinant enzyme, substrate trisialylated acceptor Campylobacter jejuni
0.0778
-
 purified recombinant enzyme, substrate disialylated acceptor Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + N-acetylneuraminic acid
-
 Campylobacter jejuni CoA + N-acetyl-9-O-acetyl-neuraminate no 7-O-acetylation ?
acetyl-CoA + NeuAcalpha-2,3-Galbeta-1,4-Glc-6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester the enzyme catalyzes the transfer of O-acetyl groups onto oligosaccharide-bound sialic acid, with a high specificity for terminal alpha2,8-linked residues, usage of 6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester-labeled oligosaccharides Campylobacter jejuni ?
-
 ?
acetyl-CoA + NeuAcalpha-2,8-NeuAcalpha-2,3-Galbeta-1,4-Glc-6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester the enzyme catalyzes the transfer of O-acetyl groups onto oligosaccharide-bound sialic acid, with a high specificity for terminal alpha2,8-linked residues, usage of 6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester-labeled oligosaccharides Campylobacter jejuni ?
-
 ?
acetyl-CoA + NeuAcalpha-2,8-NeuAcalpha-2,8-NeuAcalpha-2,3-Galbeta-1,4-Glc-6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester the enzyme catalyzes the transfer of O-acetyl groups onto oligosaccharide-bound sialic acid, with a high specificity for terminal alpha2,8-linked residues, usage of 6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester-labeled oligosaccharides Campylobacter jejuni ?
-
 ?
additional information the enzyme is involved in the lipo-oligosaccharide biosynthesis, and in production of sialylated outer core structures that mimic host gangliosides, implicated in triggering the onset of Guillain-Barré syndrome Campylobacter jejuni ?
-
 ?
additional information substrate specificity, overview, NeuAcalpha-2,3-Galalpha-1,4-Glc-6-(5-fluorescein-carboxamido)-hexanoic acid succimidyl ester is a poor substrate Campylobacter jejuni ?
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the NodL-LacA-CysE family of O-acetyltransferase family Campylobacter jejuni
sialate O-acetyltransferase
-
 Campylobacter jejuni
SOAT
-
 Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Campylobacter jejuni