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Literature summary for 2.3.1.45 extracted from
- Characterization of the enzymic 7-O-acetylation of sialic acids and evidence for enzymic O-acetyl migration from C-7 to C-9 in bovine submandibular gland (1998), J. Biochem., 124, 111-121.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slightly activates | Bos taurus |  |
| acetic acid anhydride | activates | Bos taurus | |
| dithiothreitol | slightly activates | Bos taurus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | concentration-dependent | Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| diethyl dicarbonate | inhibits intramolecular migration of the acetyl group from position 7 to 9 | Bos taurus | |
| methylamine | slightly | Bos taurus | |
| N-Acetylimidazole | - |
Bos taurus | |
| N-bromosuccinimide | slightly | Bos taurus | |
| p-chloromercuribenzoate | - |
Bos taurus | |
| Triton X-100 | above 0.02% | Bos taurus | |
| Zn2+ | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00163 | - |
acetyl-CoA | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi vesicle | - |
Bos taurus | - |
- |
| microsome | - |
Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + sialic acid | Bos taurus | - |
CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + N-acetylneuraminate = CoA + N-acetyl-7-O(or 9-O)-acetylneuraminate | 7-O-acetyl groups can migrate to the 9-position | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submandibular gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
subcellular distribution | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + N-acetylneuraminate | glycosidically bound N-acetylneuraminate | Bos taurus | CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | O-acetylation at position 7, 9-O-acetylated product appears after migration of the acetyl group from position 7 to 9 | ? | |
| acetyl-CoA + sialic acid | - |
Bos taurus | CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate | - |
? | |
| additional information | reaction affords the transport of the acetyl group by a translocase across the Golgi membrane, followed by the enzymatic transfer of the acetyl group onto sialic acid in the Golgi lumen | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0112 | - |
CoA | - |
Bos taurus | |
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