Cloned (Comment) | Organism |
---|---|
transient expression of wild-type and mutant enzymes in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E149A | site-directed mutagenesis, mutation alters the human enzyme residue to the corresponding residue of the rat sequence, 2.9fold increased cholesteryl ester formation activity, 5.5fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme | Homo sapiens |
E149A/Y292H/W294F | site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, increased cholesteryl ester formation activity and phospholipase A2 activity with 1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine, decreased activities with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine compared to the wild-type enzyme | Homo sapiens |
Y292H/W294F | site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, 1.4fold increased cholesteryl ester formation activity, 2.8fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from COS-1 cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cholesterol esterification and phospholipase A2 activities of wild-type and mutant enzymes with different substrates, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine + cholesterol | - |
Homo sapiens | ? | - |
? | |
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol | - |
Homo sapiens | ? | - |
? | |
additional information | the fatty acid substrate specificity is determined by residues E149, Y292, and W294, enzyme also shows phospholipase A2 activity with the substrates | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LCAT | - |
Homo sapiens |
lecithin: cholesterol acyltransferase | - |
Homo sapiens |