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Literature summary for 2.3.1.43 extracted from
- Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase (1999), J. Lipid Res., 40, 1512-1519.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of a C-terminal histidine tagged enzyme in Chinese hamster ovary cells | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | a recombinant C-terminal histidine tagged enzyme has similar carbohydrate moieties than the enzyme purified from plasma | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of a recombinant C-terminal histidine tagged enzyme using a cobalt affinity resin | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.43 | - |
recombinant C-terminal histidine tagged enzyme, similar to plasma enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 60 days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine | - |
Homo sapiens | cholesteryl oleate + 1-palmitoylglycerophosphocholine | - |
? |  |
| phosphatidylcholine + sterol | - |
Homo sapiens | 1-acylglycerophosphocholine + sterol ester | - |
? | |
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Release 2023.1 (January 2023)
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