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Literature summary for 2.3.1.43 extracted from
- Effects of inhibitors of N-linked oligosaccharide processing on the secretion, stability, and activity of lecithin:cholesterol acyltransferase (1991), Biochemistry, 30, 3228-3234.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| castanospermine | reduces the maximum reaction velocity but not the Km | Homo sapiens |  |
| deoxynojirimycin | reduces the maximum reaction velocity but not the Km | Homo sapiens | |
| methyldeoxynojirimycin | reduces the maximum reaction velocity but not the Km | Homo sapiens | |
| tunicamycin | reduces the maximum reaction velocity but not the Km | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.4 | - |
cholesterol | in cholesterol-lecithin vesicles | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE after cleavage of the N-linked carbohydrates | Homo sapiens |
| 67000 | - |
x * 67000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | almost all of the carbohydrate moiety of LCAT is N-linked with part of the high-mannose and part of the complex type | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation from plasma and purification by phenyl-agarose, DEAE-cellulose and hydroxyapatite columns | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| plasma | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphatidylcholine + sterol | - |
Homo sapiens | 1-acylglycerophosphocholine + sterol ester | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Homo sapiens |
| ? | x * 46000, SDS-PAGE after cleavage of the N-linked carbohydrates | Homo sapiens |
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