Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asolectin | activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol | Cavia porcellus | |
Phospholipid | crude peroxisomal phospholipids | Cavia porcellus | |
Phospholipid | at low concentration: phosphatidylserine and phosphatidylethanolamine, both inhibitory at high concentration | Cavia porcellus | |
Phospholipid | phosphatidylglycerol | Cavia porcellus | |
Phospholipid | activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine | Cavia porcellus |
General Stability | Organism |
---|---|
asolectin stabilizes against inactivation during freezing and thawing | Cavia porcellus |
DEAE-Sephacel chromatography inactivates | Cavia porcellus |
freezing inactivates | Cavia porcellus |
isoelectric focusing inactivates | Cavia porcellus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithio-bis(2-nitrobenzoate) | i.e. DTNB | Cavia porcellus | |
Cd2+ | - |
Cavia porcellus | |
cholate | above 2 mg/ml | Cavia porcellus | |
Cu2+ | - |
Cavia porcellus | |
iodoacetamide | 5 mM, weak | Cavia porcellus | |
p-chloromercuriphenylsulfonic acid | 5 mM | Cavia porcellus | |
phosphatidic acid | - |
Cavia porcellus | |
phosphatidylethanolamine | above 1.5 mM | Cavia porcellus | |
phosphatidylinositol | - |
Cavia porcellus | |
phosphatidylserine | above 1.5 mM | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Cavia porcellus | |
0.065 | - |
palmitoyl-CoA | - |
Cavia porcellus | |
0.1 | 0.5 | dihydroxyacetone phosphate | in presence of asolectin | Cavia porcellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Cavia porcellus | 5777 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by Ca2+ | Cavia porcellus | |
additional information | no activation by Mg2+, partially purified | Cavia porcellus | |
additional information | no activation by Mn2+, partially purified | Cavia porcellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, solubilized with sodium cholate-KCl | Cavia porcellus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Cavia porcellus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.106 | - |
- |
Cavia porcellus |
0.212 | - |
- |
Cavia porcellus |
Storage Stability | Organism |
---|---|
2-4°C, 30-50% loss of activity after 1 month, DTT stabilizes during storage | Cavia porcellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + dihydroxyacetone phosphate | i.e. glycerone phosphate, saturated acyl-CoAs preferred over unsaturated | Cavia porcellus | CoA + acyldihydroxyacetone phosphate | i.e. acylglycerone phosphate | r | |
arachidonoyl-CoA + dihydroxyacetone phosphate | i.e. eicosatetraenoyl-CoA, poor substrate | Cavia porcellus | CoA + arachidonoyldihydroxyacetone phosphate | - |
r | |
linoleoyl-CoA + dihydroxyacetone phosphate | - |
Cavia porcellus | CoA + linoleoyldihydroxyacetone phosphate | - |
r | |
oleoyl-CoA + dihydroxyacetone phosphate | poor substrate | Cavia porcellus | CoA + oleoyldihydroxyacetone phosphate | - |
r | |
palmitoyl-CoA + dihydroxyacetone phosphate | best substrate | Cavia porcellus | CoA + monopalmitoyldihydroxyacetone phosphate | - |
r | |
stearoyl-CoA + dihydroxyacetone phosphate | acylation at 35% the rate of palmitoyl-CoA | Cavia porcellus | CoA + stearoyldihydroxyacetone phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cavia porcellus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
t1/2: 15 min | Cavia porcellus |
51 | - |
t1/2: 15 min, with asolectin | Cavia porcellus |
60 | - |
10 min stable | Cavia porcellus |
60 | - |
intact peroxisomes | Cavia porcellus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
in presence of asolectin | Cavia porcellus |
7 | 8.2 | broad | Cavia porcellus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about half-maximal activity at pH 6.0 and pH 9.0, in the presence of asolectin | Cavia porcellus |