BRENDA - Enzyme Database show
show all sequences of 2.3.1.41

Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis

Clough, R.; Matthis, A.L.; Barnum, S.R.; Jaworski, J.G.; J. Biol. Chem. 267, 20992-20998 (1992)

Data extracted from this reference:

General Stability
General Stability
Organism
dithiothreitol stabilizes
Spinacia oleracea
glycerol stabilizes
Spinacia oleracea
Triton X-100 stabilizes
Spinacia oleracea
Inhibitors
Inhibitors
Commentary
Organism
Structure
arsenite
synthase III
Spinacia oleracea
N-ethylmaleimide
-
Spinacia oleracea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
2 * 40500, synthase III, SDS-PAGE
Spinacia oleracea
63000
-
synthase III, gel filtration
Spinacia oleracea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Spinacia oleracea
-
synthase III
-
Purification (Commentary)
Commentary
Organism
synthase III, native wild-type and recombinant from E. coli E103S
Spinacia oleracea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
comparison of activity, recombinant and native enzyme
Spinacia oleracea
7
-
purified native enzyme
Spinacia oleracea
Storage Stability
Storage Stability
Organism
-80░C, several weeks in the presence of 20% v/v glycerol, 0.1% Triton X-100 and dithiothreitol
Spinacia oleracea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase III, recombinant enzyme from E. coli is absolutely specific for
486931
Spinacia oleracea
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486931
Spinacia oleracea
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486931
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486931
Spinacia oleracea
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 40500, synthase III, SDS-PAGE
Spinacia oleracea
General Stability (protein specific)
General Stability
Organism
dithiothreitol stabilizes
Spinacia oleracea
glycerol stabilizes
Spinacia oleracea
Triton X-100 stabilizes
Spinacia oleracea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
arsenite
synthase III
Spinacia oleracea
N-ethylmaleimide
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
2 * 40500, synthase III, SDS-PAGE
Spinacia oleracea
63000
-
synthase III, gel filtration
Spinacia oleracea
Purification (Commentary) (protein specific)
Commentary
Organism
synthase III, native wild-type and recombinant from E. coli E103S
Spinacia oleracea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
comparison of activity, recombinant and native enzyme
Spinacia oleracea
7
-
purified native enzyme
Spinacia oleracea
Storage Stability (protein specific)
Storage Stability
Organism
-80░C, several weeks in the presence of 20% v/v glycerol, 0.1% Triton X-100 and dithiothreitol
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase III, recombinant enzyme from E. coli is absolutely specific for
486931
Spinacia oleracea
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486931
Spinacia oleracea
r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486931
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486931
Spinacia oleracea
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40500, synthase III, SDS-PAGE
Spinacia oleracea
Other publictions for EC 2.3.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736660
Sabbagh
Docking studies of flavonoid c ...
Escherichia coli
J. Mol. Graph. Model.
61
214-223
2015
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737010
Yuan
-
Cloning and function character ...
Cocos nucifera
Plant Mol. Biol. Rep.
33
1131-1140
2015
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1
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1
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737055
Ding
OsKASI, a beta-ketoacyl-[acyl ...
Oryza sativa
Planta
242
203-213
2015
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1
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1
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1
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3
3
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736441
Kapilashrami
Thiolactomycin-based beta-keto ...
Mycobacterium tuberculosis
J. Biol. Chem.
288
6045-6052
2013
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1
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1
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19
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1
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1
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1
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17
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1
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19
17
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1
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1
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1
1
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718505
Doan
Crystallization and preliminar ...
Xanthomonas oryzae pv. oryzae
Acta Crystallogr. Sect. F
67
1548-1550
2011
-
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1
1
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1
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718888
Borgaro
Substrate recognition by beta- ...
Escherichia coli, Mycobacterium tuberculosis
Biochemistry
50
10678-10686
2011
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2
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5
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1
18
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2
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2
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12
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18
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18
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12
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18
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17
17
719202
Lee
-
In silico screening of a novel ...
Escherichia coli
Bull. Korean Chem. Soc.
32
1645-1649
2011
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1
1
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4
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2
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1
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1
2
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6
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2
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1
1
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720679
Wu
Arabidopsis beta-ketoacyl-[acy ...
Arabidopsis thaliana
Plant Cell
22
3726-3744
2010
-
-
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-
-
-
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1
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1
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704553
Feng
Escherichia coli unsaturated f ...
Escherichia coli
J. Biol. Chem.
284
29526-29535
2009
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1
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2
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1
1
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674882
Stephens
Mitochondrial fatty acid synth ...
Trypanosoma brucei
J. Biol. Chem.
282
4427-4436
2007
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1
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2
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1
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2
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1
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1
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676903
Wang
Discovery of platencin, a dual ...
Staphylococcus aureus
Proc. Natl. Acad. Sci. USA
104
7612-7616
2007
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1
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2
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1
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1
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677030
Christensen
Structure of the human beta-ke ...
Homo sapiens
Protein Sci.
16
261-272
2007
-
1
1
1
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2
-
2
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8
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1
1
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1
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1
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673576
von Wettstein-Knowles
Fatty acid synthesis. Role of ...
Escherichia coli
FEBS J.
273
695-710
2006
-
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1
8
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3
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8
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1
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2
1
1
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674777
Lack
Recombinant expression and bio ...
Plasmodium falciparum
J. Biol. Chem.
281
9538-9546
2006
-
1
1
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2
7
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1
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5
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1
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2
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8
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1
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12
1
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1
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2
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1
1
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2
2
1
7
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1
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1
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2
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8
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1
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12
1
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659451
Zhang
Cloning, expression, and chara ...
Homo sapiens
J. Biol. Chem.
280
12422-12429
2005
-
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1
7
3
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1
1
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5
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1
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8
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10
1
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6
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1
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7
3
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1
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8
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10
1
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6
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659493
Brown
Probing the mechanism of the M ...
Mycobacterium tuberculosis
J. Biol. Chem.
280
32539-32547
2005
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6
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1
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659724
Musayev
Crystal structure of a substra ...
Mycobacterium tuberculosis
J. Mol. Biol.
346
1313-1321
2005
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3
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659726
Kursula
High resolution crystal struct ...
Escherichia coli
J. Mol. Biol.
347
189-201
2005
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4
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660466
Qiu
Crystal structure and substrat ...
Staphylococcus aureus
Protein Sci.
14
2087-2094
2005
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1
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1
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661000
Rendina
Characterization of the inacti ...
Rattus norvegicus
Biochem. J.
388
895-903
2005
-
-
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2
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2
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1
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1
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2
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1
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1
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1
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1
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674095
Salas
Very long chain fatty acid syn ...
Helianthus annuus
J. Agric. Food Chem.
53
2710-2716
2005
-
-
-
-
-
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1
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2
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1
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3
-
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1
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1
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1
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1
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1
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1
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2
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1
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658746
Olsen
Structure of the mitochondrial ...
Arabidopsis thaliana
FEBS Lett.
577
170-174
2004
-
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1
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1
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1
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5
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1
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1
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659442
Yasuno
Identification and molecular c ...
Arabidopsis thaliana
J. Biol. Chem.
279
8242-8251
2004
-
-
1
-
-
-
1
-
2
-
1
-
-
4
-
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-
-
-
-
-
-
659029
Price
The 1.3-Angstrom-resolution cr ...
Streptococcus pneumoniae
J. Bacteriol.
185
4136-4143
2003
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
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-
-
-
-
-
-
659294
Lai
beta-Ketoacyl-acyl carrier pro ...
Lactococcus lactis
J. Biol. Chem.
278
51494-51503
2003
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
-
-
-
-
-
-
660470
Dawe
A template search reveals mech ...
Escherichia coli
Proteins
52
427-435
2003
-
-
-
-
-
-
-
-
-
-
-
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486944
Witkowski
Mechanism of the beta-ketoacyl ...
Rattus norvegicus
Biochemistry
41
10877-10887
2002
-
-
-
-
2
-
1
1
-
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2
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486946
He
Purification, characterization ...
Staphylococcus aureus
Antimicrob. Agents Chemother.
46
1310-1318
2002
-
1
1
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-
4
3
-
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3
2
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1
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486947
Schutt
beta-Ketoacyl-acyl carrier pro ...
Cuphea lanceolata
Planta
215
847-854
2002
-
-
1
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1
1
-
-
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1
-
5
-
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-
-
486938
Smirnova
Engineered fatty acid biosynth ...
Streptomyces glaucescens
J. Bacteriol.
183
2335-2342
2001
-
-
1
-
3
-
2
-
-
-
-
1
-
1
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1
1
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6
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-
-
-
486939
Khandekar
Identification, substrate spec ...
Escherichia coli, Haemophilus influenzae, Streptococcus pneumoniae
J. Biol. Chem.
276
30024-30030
2001
-
-
2
-
-
-
9
2
-
-
4
-
-
3
-
-
2
3
-
-
1
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8
-
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8
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-
486940
Moche
The crystal structure of beta- ...
Synechocystis sp.
J. Mol. Biol.
305
491-503
2001
-
3
1
1
-
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-
-
-
-
-
-
1
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486941
Price
Inhibition of beta-ketoacyl-ac ...
Escherichia coli
J. Biol. Chem.
276
6551-6559
2001
-
-
1
1
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2
-
-
-
-
-
-
1
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486942
Schaeffer
Purification and biochemical c ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Biol. Chem.
276
47029-47037
2001
-
1
1
-
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-
2
5
-
-
-
1
-
5
-
-
1
-
-
-
4
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17
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17
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2
2
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6
2
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-
486943
McGuire
beta-Ketoacyl-[acyl carrier pr ...
Escherichia coli
Biochemistry
40
9836-9845
2001
-
-
1
-
7
-
1
-
-
-
1
-
-
2
-
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1
1
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1
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-
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486948
Mekhedov
An unusual seed-specific 3-ket ...
Coriandrum sativum
Plant Mol. Biol.
47
507-518
2001
-
-
1
-
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-
1
-
1
-
1
1
-
7
-
-
1
1
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1
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1
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3
-
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1
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-
486935
Domergue
Biochemical characterization o ...
Allium ampeloprasum
Biochem. Soc. Trans.
28
610-613
2000
-
-
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1
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-
3
-
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1
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486936
Val
Re-engineering ketoacyl syntha ...
Synechocystis sp.
Structure
8
565-566
2000
-
3
1
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2
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1
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-
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3
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486937
Choi
beta-ketoacyl-acyl carrier pro ...
Bacillus subtilis, Escherichia coli
J. Bacteriol.
182
365-370
2000
-
-
1
-
-
-
-
-
-
-
4
2
-
2
-
-
1
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2
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6
1
2
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2
-
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1
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2
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2
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6
1
2
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-
2
-
-
-
-
-
-
-
-
-
486934
Bao
Purification and properties of ...
Streptomyces peucetius
Biochemistry
38
9752-9757
1999
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
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1
1
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-
-
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-
-
-
-
-
-
-
-
-
486933
Han
Characterization of beta-ketoa ...
Streptomyces glaucescens
J. Bacteriol.
180
4481-4486
1998
-
-
1
-
-
-
1
4
-
-
2
1
-
1
-
-
1
-
-
-
-
-
7
1
1
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2
-
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1
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1
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4
-
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2
1
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1
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7
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
486932
Verwoert
Modification of Brassica napus ...
Brassica napus, Escherichia coli
Plant Mol. Biol.
27
875-886
1995
-
-
1
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
2
-
4
-
-
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-
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1
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2
-
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2
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2
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486931
Clough
Purification and characterizat ...
Spinacia oleracea
J. Biol. Chem.
267
20992-20998
1992
-
-
-
-
-
3
2
-
-
-
2
-
-
2
-
-
1
-
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1
2
1
2
1
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-
-
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-
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-
-
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-
3
-
2
-
-
-
-
2
-
-
-
-
1
-
1
2
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486925
Siggaard-Andersen
Primary structure of a cerulen ...
Hordeum vulgare, Spinacia oleracea
Proc. Natl. Acad. Sci. USA
88
4114-4118
1991
-
-
1
-
-
-
-
-
2
-
5
-
-
5
-
-
1
-
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
1
-
2
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486922
MacKintosh
-
beta-Ketoacyl-acyl-carrier pro ...
Brassica napus
Biochem. Soc. Trans.
17
686-687
1989
-
-
-
-
-
1
-
-
-
-
3
1
-
1
-
-
1
-
-
1
2
-
2
1
-
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-
-
-
-
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-
-
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-
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-
-
-
-
-
1
-
-
-
-
-
-
3
1
-
-
-
1
-
1
2
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2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486923
MacKintosh
-
A new assay procedure to study ...
Brassica napus
Biochim. Biophys. Acta
1002
114-124
1989
-
-
-
-
-
3
1
-
-
-
3
-
-
1
-
-
1
-
-
1
1
1
4
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
3
-
-
-
-
1
-
1
1
1
4
1
1
-
-
-
1
2
-
-
-
-
-
-
-
-
285675
Shimakata
The purification and function ...
Brassica juncea, Carthamus tinctorius, Cuphea lutea, Pisum sativum, Spinacia oleracea
J. Biol. Chem.
258
3592-3598
1983
-
-
-
-
-
-
1
-
-
-
2
-
-
7
-
-
1
-
-
8
4
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
8
4
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486920
Shimakata
Purification and characterizat ...
Spinacia oleracea
Arch. Biochem. Biophys.
220
39-45
1983
-
-
-
-
-
-
4
5
-
-
1
-
-
2
-
-
1
-
-
1
1
1
7
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
5
-
-
1
-
-
-
-
1
-
1
1
1
7
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
285669
Shimakata
The procaryotic nature of the ...
Carthamus tinctorius
Arch. Biochem. Biophys.
217
144-154
1982
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486921
Schuz
-
Partial purification of beta-k ...
Petroselinum crispum
FEBS Lett.
140
207-209
1982
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
486924
Shimakata
Isolation and function of spin ...
Spinacia oleracea
Proc. Natl. Acad. Sci. USA
79
5808-5812
1982
-
-
-
-
-
-
4
4
-
-
2
2
-
2
-
-
1
-
-
1
1
1
11
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
4
-
-
2
2
-
-
-
1
-
1
1
1
11
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
285676
Hendren
Fatty acid synthetases from Eu ...
Euglena gracilis, Euglena gracilis Z
J. Biol. Chem.
255
1504-1508
1980
-
-
-
-
-
1
-
-
1
-
1
-
-
22
-
-
1
-
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
486916
Garwin
Structural, enzymatic, and gen ...
Escherichia coli
J. Biol. Chem.
255
11949-11956
1980
2
-
-
-
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1
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3
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1
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1
1
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2
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8
1
2
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-
-
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-
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-
-
2
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-
-
-
-
-
-
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1
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-
3
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1
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2
-
8
1
2
-
-
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-
-
-
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-
486918
D'Agnolo
beta-Ketoacyl-acyl carrier pro ...
Escherichia coli
J. Biol. Chem.
250
5283-5288
1975
2
-
-
-
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-
1
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-
-
-
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-
2
1
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1
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-
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3
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2
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1
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-
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1
-
1
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-
-
-
3
-
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-
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486919
D'Agnolo
Multiple forms of beta-ketoacy ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
250
5289-5294
1975
-
-
-
-
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1
6
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-
2
2
-
178
-
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1
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2
-
15
-
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1
-
2
2
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-
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1
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6
-
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2
2
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1
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-
2
-
15
-
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1
-
2
2
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-
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486863
Vagelos
-
Acyl group transfer (acyl carr ...
Clostridium kluyveri, Escherichia coli
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
8
155-199
1973
3
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1
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3
1
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3
4
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2
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1
1
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1
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17
3
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1
1
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3
-
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1
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3
-
1
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3
4
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1
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1
-
17
3
-
-
-
-
1
1
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-
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-
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486864
Prescott
Acyl carrier protein ...
Escherichia coli
Adv. Enzymol. Relat. Areas Mol. Biol.
36
269-311
1972
2
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1
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-
2
1
1
-
1
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1
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1
1
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2
4
2
1
-
1
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2
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1
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2
-
1
1
-
1
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1
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-
2
4
2
1
-
1
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486917
Alberts
Acyl carrier protein. XV. Stud ...
Escherichia coli
J. Biol. Chem.
247
3190-3198
1972
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1
1
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1
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1
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1
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11
-
1
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1
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1
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1
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1
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11
-
1
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486915
Alberts
-
beta-Ketoacyl acyl carrier pro ...
Escherichia coli
Methods Enzymol.
14
57-60
1969
3
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2
2
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2
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1
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1
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1
1
4
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1
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1
1
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3
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2
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2
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2
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1
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1
1
4
-
1
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1
1
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486914
Toomey
Studies on the mechanism of fa ...
Escherichia coli
J. Biol. Chem.
241
1159-1165
1966
1
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3
4
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1
2
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1
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1
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1
1
8
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1
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1
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1
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3
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4
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1
2
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1
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1
1
8
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1
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1
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