BRENDA - Enzyme Database
show all sequences of 2.3.1.40

The incorporation of beta-hydroxy fatty acids into a phospholipid of Escherichia coli B

Taylor, S.S.; Heath, E.C.; J. Biol. Chem. 244, 6605-6616 (1969)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Escherichia coli
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, 0.2 M
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
B
-
Escherichia coli B / ATCC 11303
-
B
-
Purification (Commentary)
Commentary
Organism
partial, solubilization by sonic disruption
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli
?
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli B / ATCC 11303
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli B / ATCC 11303
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
inactivation after 2-5 min
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
10
about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no stimulation by coenzyme A, ATP, GTP, UTP, glucosamine 1-phosphate, UDP-N-acetylglucosamine, Triton X-100, lipid A, alkaline treated lipid A or alkaline treated lipid A annealed with Escherichia coli phospholipids
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
particle-bound
-
Escherichia coli
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activation, 0.2 M
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
partial, solubilization by sonic disruption
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli
?
acyl-(acyl-carrier protein) + O-(2-acyl-sn-glycero-3-phospho) ethanolamine
specific for C1-position of glycerol-phosphate backbone, the acyl-component of the acyl-(acyl-carrier-protein) may be 3-hydroxydecanoate, 3-hydroxylaurate, 3-hydroxymyristate and 3-hydroxyoctanoate (tentatively identified)
486910
Escherichia coli B / ATCC 11303
(acyl-carrier protein) + O-(1,2-diacyl-sn-glycero-3-phospho) ethanolamine
-
486910
Escherichia coli B / ATCC 11303
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Escherichia coli
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
inactivation after 2-5 min
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
10
about half-maximal activity at pH 5.0, about 60% of maximal activity at pH 10.0
Escherichia coli
Other publictions for EC 2.3.1.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
486913
Heath
A conserved histidine is essen ...
Escherichia coli
J. Bacteriol.
180
1425-1430
1998
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1
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1
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1
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1
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486912
Jackowski
Sequence and function of the a ...
Escherichia coli
J. Biol. Chem.
269
2921-2928
1994
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1
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1
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3
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1
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1
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486911
Nishida
-
Purification of isomeric forms ...
Cucurbita moschata
Plant Cell Physiol.
28
1071-1079
1987
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1
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3
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1
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486910
Taylor
The incorporation of beta-hydr ...
Escherichia coli, Escherichia coli B / ATCC 11303
J. Biol. Chem.
244
6605-6616
1969
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177
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