Application | Comment | Organism |
---|---|---|
molecular biology | a microtiter plate based assay to detect the GlmU activity is developed: the assay relies on the enzymes MurA and MurB to convert UDP-GlcNAc into UDP-MurNAc with the concomitant reduction of NADPH. When all enzymes and substrates are present NADPH is oxidized with a concomitant decrease in OD340 | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a GlmU knockout strain discloses that GlmU is required for growth of Mycobacterium smegmatis as the bacteria does not grow in the absence of active GlmU enzyme | Mycolicibacterium smegmatis |
additional information | in a temperature shift experiment (from 30°C to 42°C) the amount of functional GlmU enzyme is gradually reduced the Mycobacterium smegmatis cells become non-viable and their morphology change from a normal rod shape to stubby-rounded morphology and in some cases they lyse | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54100 | - |
SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a HIS-Select HF affinity gel column | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + D-glucosamine 6-phosphate | - |
Mycolicibacterium smegmatis | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? | |
acetyl-CoA + D-glucosamine 6-phosphate | - |
Mycobacterium tuberculosis | CoA + N-acetyl-D-glucosamine 6-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlmU | GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities | Mycolicibacterium smegmatis |
GlmU | GlmU protein exhibits both glucosamine-1-phosphate acetyltransferase and N-acetylglucosamine-1-phosphate uridylyltransferase activities | Mycobacterium tuberculosis |
glucosamine-6-phosphate N-acetyltransferase | - |
Mycolicibacterium smegmatis |
glucosamine-6-phosphate N-acetyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay carried out at room temperature | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Mycobacterium tuberculosis |