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Literature summary for 2.3.1.39 extracted from

  • Lee, W.; Park, J.; Balasubramanian, P.; Kim, Y.
    Elucidation of the crystal structure of FabD from the multidrug-resistant bacterium Acinetobacter baumannii (2018), Biochem. Biophys. Res. Commun., 505, 208-214 .
    View publication on PubMed
Search for more literature for 2.3.1.39

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter baumannii
subcloned into NdeI and XhoI sites of the pET28a expression vector, and transformed into Escherichia coli BL21 (DE3) Acinetobacter baumannii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Acinetobacter baumannii
sitting drop vapor diffusion method, using 1.4 M ammonium tartrate dibasic and 0.1 M Tris (pH 8.5) Acinetobacter baumannii

Protein Variants

Protein Variants Comment Organism
K195A/K200A the mutation results in a dramatic decrease in acyl-carrier protein binding Acinetobacter baumannii

Inhibitors

Inhibitors Comment Organism Structure
Trifluoperazine
-
 Acinetobacter baumannii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + an [acyl-carrier protein] Acinetobacter baumannii
-
 CoA + a malonyl-[acyl-carrier protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter baumannii V5VFX0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acinetobacter baumannii
HiTrap chelating Sepharose column chromatography Acinetobacter baumannii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + an [acyl-carrier protein]
-
 Acinetobacter baumannii CoA + a malonyl-[acyl-carrier protein]
-
 ?

Synonyms

Synonyms Comment Organism
FabD
-
 Acinetobacter baumannii
malonyl CoA-ACP transacylase
-
 Acinetobacter baumannii