Literature summary for 2.3.1.39 extracted from

Marcella, A.M.; Barb, A.W.
The R117A variant of the Escherichia coli transacylase FabD synthesizes novel acyl-(acyl carrier proteins) (2017), Appl. Microbiol. Biotechnol., 101, 8431-8441 .

Protein Variants

Protein Variants	Comment	Organism
R117A	the variant synthesizes acetyl-[acyl carrier protein], succinyl-[acyl carrier protein], isobutyryl-[acyl carrier protein], 2-butenoyl-[acyl carrier protein], and beta-hydroxybutyryl-[acyl carrier protein] among others from holo-acyl carrier protein and the corresponding acyl-CoAs	Escherichia coli
R117A	unlike the wild-type enzyme that is highly specific for malonyl-CoA to produce malonyl-[acyl-carrier protein] (ACP), the R117A variant synthesizes acetyl-ACP, succinyl-ACP, isobutyryl-ACP, 2-butenoyl-ACP, and beta-hydroxybutyryl-ACP among others from holo-ACP and the corresponding acyl-CoAs with specific activities from 3.7 to 120 nmol/min*mg	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.011	-	an [acyl-carrier protein]	mutant enzyme R117A, with methylmalonyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.02	-	an [acyl-carrier protein]	wild type enzyme, with methylmalonyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.03	-	an [acyl-carrier protein]	mutant enzyme R117A, with acetoacetyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.03	-	an [acyl-carrier protein]	mutant enzyme R117A, with malonyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.04	-	an [acyl-carrier protein]	mutant enzyme R117A, with acetyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.04	-	an [acyl-carrier protein]	wild type enzyme, with malonyl-CoA as cosubstrate, pH and temperature not specified in the publication	Escherichia coli
0.06	-	malonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Escherichia coli
0.06	-	malonyl-CoA	wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli
0.08	-	malonyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.11	-	acetoacetyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.2	-	acetyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.33	-	methylmalonyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.58	-	methylmalonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Escherichia coli
0.58	-	methylmalonyl-CoA	wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
malonyl-CoA + an [acyl-carrier protein]	Escherichia coli	-	CoA + a malonyl-[acyl-carrier protein]	-	?
malonyl-CoA + an [acyl-carrier protein]	Escherichia coli	the wild type enzyme is highly specific for malonyl-CoA	CoA + a malonyl-[acyl-carrier protein]	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.001	-	mutant enzyme R117A with isovaleryl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.0016	-	substrate: acetoacetyl-CoA, wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli
0.0016	-	wild type enzyme with acetoacetyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.0037	-	mutant enzyme R117A with isobutyryl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.005	-	mutant enzyme R117A with 2-hydroxybutyryl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.0054	-	mutant enzyme R117A with 2-butenoyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.011	-	mutant enzyme R117A with butyryl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.016	-	mutant enzyme R117A with succinyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.052	-	mutant enzyme R117A with propionyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.06	-	mutant enzyme R117A with acetoacetyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.06	-	substrate: acetoacetyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.12	-	mutant enzyme R117A with acetyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
0.12	-	substrate: acetyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
3.2	-	mutant enzyme R117A with methylmalonyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
3.2	-	substrate: methylmalonyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
13	-	mutant enzyme R117A with malonyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
13	-	substrate: malonyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
270	-	substrate: methylmalonyl-CoA, wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli
270	-	wild type enzyme with methylmalonyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli
4100	-	substrate: malonyl-CoA, wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli
4100	-	wild type enzyme with malonyl-CoA as substrate, pH and temperature not specified in the publication	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
2-butenoyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + 2-butenoyl-[acyl carrier protein]	-	?
2-hydroxybutyryl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + 2-hydroxybutyryl-[acyl carrier protein]	-	?
acetoacetyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by the wild type enzyme (very weak activity) and mutant enzyme R117A	Escherichia coli	CoA + acetoacetyl-[acyl carrier protein]	-	?
acetoacetyl-CoA + an [acyl-carrier protein]	-	Escherichia coli	CoA + an acetoacetyl-[acyl-carrier protein]	-	?
acetyl-CoA + an [acyl-carrier protein]	activity with mutant enzyme R117A, no activity with wild-type enzyme	Escherichia coli	CoA + an acetyl-[acyl-carrier protein]	-	?
acetyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + acetyl-[acyl carrier protein]	-	?
butyryl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + butyryl-[acyl carrier protein]	-	?
isobutyryl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + isobutyryl-[acyl carrier protein]	-	?
isovaleryl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + isovaleryl-[acyl carrier protein]	-	?
malonyl-CoA + an [acyl-carrier protein]	-	Escherichia coli	CoA + a malonyl-[acyl-carrier protein]	-	?
malonyl-CoA + an [acyl-carrier protein]	the wild type enzyme is highly specific for malonyl-CoA	Escherichia coli	CoA + a malonyl-[acyl-carrier protein]	-	?
malonyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by the wild type enzyme and mutant enzyme R117A	Escherichia coli	CoA + a malonyl-[acyl-carrier protein]	-	?
methylmalonyl-CoA + an [acyl-carrier protein]	-	Escherichia coli	CoA + methylmalonyl-[acyl-carrier protein]	-	?
methylmalonyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by the wild type enzyme (low activity) and mutant enzyme R117A	Escherichia coli	CoA + methylmalonyl-[acyl carrier protein]	-	?
additional information	the wild type enzyme shows no activity with acetyl-CoA, propionyl-CoA, succinyl-CoA, butyryl-CoA, 2-butenoyl-CoA, 2-hydroxybutyryl-CoA, isobutyryl-CoA, and isovaleryl-CoA	Escherichia coli	?	-	-
propionyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + propionyl-[acyl carrier protein]	-	?
succinyl-CoA + an [acyl-carrier protein]	the reaction is catalyzed by mutant enzyme R117A	Escherichia coli	CoA + succinyl-[acyl carrier protein]	-	?

Synonyms

Synonyms	Comment	Organism
FabD	-	Escherichia coli
malonyl-CoA/holo-ACP transacylase	-	Escherichia coli
malonyl-coenzyme A (CoA)/holo-ACP transacylase	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.029	-	acetyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
0.041	-	acetoacetyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
5	-	methylmalonyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
8.5	-	malonyl-CoA	mutant enzyme R117A, pH and temperature not specified in the publication	Escherichia coli
1000	-	methylmalonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Escherichia coli
1000	-	methylmalonyl-CoA	wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli
2100	-	malonyl-CoA	wild type enzyme, pH and temperature not specified in the publication	Escherichia coli
2100	-	malonyl-CoA	wild-type enzyme, pH and temperature not specified in the publication	Escherichia coli