Cloned (Comment) | Organism |
---|---|
gene fabD, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
F187A | site-directed mutagenesis, the mutant shows loss of about 65-70% activity compared to the wild-type enzyme | Synechocystis sp. |
H188A | site-directed mutagenesis, inactive mutant | Synechocystis sp. |
H87A | site-directed mutagenesis, the mutant shows loss of about 80% activity compared to the wild-type enzyme | Synechocystis sp. |
Q10A | site-directed mutagenesis, the mutant shows loss of about 70% activity compared to the wild-type enzyme | Synechocystis sp. |
R113A | site-directed mutagenesis, inactive mutant | Synechocystis sp. |
S88T | site-directed mutagenesis, the mutant initializes the reaction by H87 deprotonating S88T which is different from the wild-type, loss of 10% activity compared to the wild-type enzyme | Synechocystis sp. |
T56A | site-directed mutagenesis, the mutant shows loss of about 60% activity compared to the wild-type enzyme | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] | Synechocystis sp. | - |
CoA + a malonyl-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | P73242 | gene fabD or slr2023 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | residues Arg113, Ser88 and His188 constitute the enzyme's catalytic triad, catalytic mechanism, detailed overview. Malonyl-CoA binds to MCAT, involving Ser88, Arg113, Met117 and Phe187. The second step forming an ACP-MCAT-malonyl intermediate is rate-limiting instead of the malonyl-CoA-MCAT intermediate formed in the first step. His87, Arg113 and Ser88 render different contributions for the two intermediates | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] | - |
Synechocystis sp. | CoA + a malonyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-coenzyme A: acyl-carrier protein transacylase | - |
Synechocystis sp. |
MCAT | - |
Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
additional information | residues Arg113, Ser88 and His188 constitute cthe enzyme's atalytic triad. Structure homology modeling by molecular replacement using the enzyme from Escherichia coli, PDB: 1MLA, as a search model | Synechocystis sp. |