Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Eimeria tenella |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
corytuberine | - |
Eimeria tenella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0228 | - |
malonyl-CoA | pH 6.8, 28°C | Eimeria tenella |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Eimeria tenella | 20011 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38800 | - |
calculated from cDNA | Eimeria tenella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eimeria tenella | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | computer analysis predicts that EtMCAT protein contains a 20 aa N-terminal signal peptide plus 37 aa transit peptide for targeting and translocating into the apicoplast | Eimeria tenella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH | Eimeria tenella | CoA + malonyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EtMCAT | - |
Eimeria tenella |
FabD | - |
Eimeria tenella |
malonyl-CoA:acyl carrier protein transacylase | - |
Eimeria tenella |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Eimeria tenella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Eimeria tenella |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01647 | - |
pH 6.8, 28°C | Eimeria tenella | corytuberine |
General Information | Comment | Organism |
---|---|---|
malfunction | functional replacement of the fabD gene with amber mutation of Escherichia coli temperature-sensitive LA2-89 strain by Eimeria tenella EtMCAT demonstrates that EcFabD and EtMCAT perform the same biochemical function | Eimeria tenella |