Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.39 extracted from

  • Hong, S.K.; Kim, K.H.; Park, J.K.; Jeong, K.W.; Kim, Y.; Kim, E.E.
    New design platform for malonyl-CoA-acyl carrier protein transacylase (2010), FEBS Lett., 584, 1240-1244.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Streptococcus pneumoniae
expressed in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively Streptococcus pneumoniae
crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively. In the SaMCAT structure, the N-terminal expression peptide of a neighboring molecule running in the opposite direction of malonyl-CoA makes extensive interactions with the highly conserved Gly-Gln-Gly-Ser-Gln stretch, suggesting a new design platform Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
F198S mutant shows a reduction in activity Streptococcus pneumoniae
H199A mutant shows a drastic reduction in activity Streptococcus pneumoniae
L89A mutant shows almost the same activity as wild-type Streptococcus pneumoniae
N158A mutant shows almost the same activity as wild-type Streptococcus pneumoniae
R115A mutant shows a reduction in activity Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus Q99UN8
-
-
Streptococcus pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Streptococcus pneumoniae CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Staphylococcus aureus CoA + malonyl-[acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
malonyl-CoA-acyl carrier protein transacylase
-
Streptococcus pneumoniae
malonyl-CoA-acyl carrier protein transacylase
-
Staphylococcus aureus
SaMCAT
-
Staphylococcus aureus
SpnMCAT
-
Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Streptococcus pneumoniae
6.8
-
assay at Staphylococcus aureus