Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Streptococcus pneumoniae |
expressed in Escherichia coli | Staphylococcus aureus |
Crystallization (Comment) | Organism |
---|---|
crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively | Streptococcus pneumoniae |
crystal structures of MCAT from Staphylococcus aureus and Streptococcus pneumoniae are determined at 1.46 and 2.1 A resolution, respectively. In the SaMCAT structure, the N-terminal expression peptide of a neighboring molecule running in the opposite direction of malonyl-CoA makes extensive interactions with the highly conserved Gly-Gln-Gly-Ser-Gln stretch, suggesting a new design platform | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
F198S | mutant shows a reduction in activity | Streptococcus pneumoniae |
H199A | mutant shows a drastic reduction in activity | Streptococcus pneumoniae |
L89A | mutant shows almost the same activity as wild-type | Streptococcus pneumoniae |
N158A | mutant shows almost the same activity as wild-type | Streptococcus pneumoniae |
R115A | mutant shows a reduction in activity | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | Q99UN8 | - |
- |
Streptococcus pneumoniae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH | Streptococcus pneumoniae | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH | Staphylococcus aureus | CoA + malonyl-[acyl-carrier protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA-acyl carrier protein transacylase | - |
Streptococcus pneumoniae |
malonyl-CoA-acyl carrier protein transacylase | - |
Staphylococcus aureus |
SaMCAT | - |
Staphylococcus aureus |
SpnMCAT | - |
Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Streptococcus pneumoniae |
6.8 | - |
assay at | Staphylococcus aureus |