Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.39 extracted from

  • Keatinge-Clay, A.T.; Shelat, A.A.; Savage, D.F.; Tsai, S.C.; Miercke, L.J.; O'Connell, J.D., 3rd; Khosla, C.; Stroud, R.M.
    Catalysis, specificity, and ACP docking site of Streptomyces coelicolor malonyl-CoA:ACP transacylase (2003), Structure, 11, 147-154.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene fabD, expression of His-tagged enzyme in Escherichia coli strain BL21 Streptomyces coelicolor

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant purified enzyme, 3 mg/ml protein in 10 mM Tris-HCl, pH 7.4, 1 mM 2-mercaptoethanol, hanging drop vapoir diffusion method, 3 days, room temperature, soaking of crystals in 30% PEG 4000, 100 mM sodium acetate, pH 4.8, 200 mM ammonium acetate, and 20% glycerol for cryoprotection, X-ray diffraction structure determination and analysis at 2.0 A resolution, macromolecular docking simulation with K47A/K190A/R287A/K293A mutant actinorhodin ACP structure, overview Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonyl-CoA + [acyl-carrier protein] Streptomyces coelicolor enzyme is involved in both fatty acid and polyketide synthesis pathways CoA + malonyl-[acyl-carrier protein]
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
gene fabD
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography to homogeneity Streptomyces coelicolor

Reaction

Reaction Comment Organism Reaction ID
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] catalytic mechanism, [acyl-carrier protein] binding site, residues Phe200 and Met126 are involved Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + [acyl-carrier protein]
-
Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?
malonyl-CoA + [acyl-carrier protein] enzyme is involved in both fatty acid and polyketide synthesis pathways Streptomyces coelicolor CoA + malonyl-[acyl-carrier protein]
-
?

Synonyms

Synonyms Comment Organism
malonyl-CoA:ACP transacylase
-
Streptomyces coelicolor
MAT
-
Streptomyces coelicolor